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Nucleic Acids Research, Vol 25, Issue 10 2039-2040, Copyright © 1997 by Oxford University Press

ARTICLES

Yeast colony size reflects YAC copy number

AV Popov, C Butzler and M Bruggemann
Department of Development and Genetics, The Babraham Institute, Cambridge CB2 4AT, UK. andrei.popov@bbsrc.ac.uk

A novel strategy for separation of co-cloned YACs was developed. For this, yeast cells were grown under non-selective conditions to allow the mitotic loss of multiple YACs. Yeast colonies of different size appear on 'drop-out' selection plates with small clones consistently containing a single-copy YAC. Different auxotrophic marker genes can be used to separate co-cloned YACs or reduce their copy number, which is essential for most YAC-modification procedures.
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