Nucleic Acids Research, Vol 25, Issue 11 2138-2146, Copyright © 1997 by Oxford University Press
J Willson, S Wilson, N Warr and FZ Watts
Checkpoint controls exist in eukaryotic cells to ensure that cells do not
enter mitosis in the presence of DNA damage or unreplicated chromosomes. In
Schizosaccharomyces pombe many of the checkpoint genes analysed to date are
required for both the DNA damage and the replication checkpoints, an
exception being chk1 . We report here on the characterization of nine new
methylmethane sulphonate (MMS)- sensitive S.pombe mutants, one of which is
defective in the DNA damage checkpoint but not the replication checkpoint.
We have cloned and sequenced the corresponding gene. The predicted protein
is most similar to the Saccharomyces cerevisiae Rad9 protein, having 46%
similarity and 26% identity. The S.pombe protein, which we have named Rhp9
(Rad9 homologue in S. pombe) on the basis of structural and phenotypic
similarity, also contains motifs present in BRCA1 and 53BP1. Deletion of
the gene is not lethal and results in a DNA damage checkpoint defect.
Epistasis analysis with other S.pombe checkpoint mutants indicates that
rhp9 acts in a process involving the checkpoint rad genes and that the rhp9
mutant is phenotypically very similar to chk1.
ARTICLES
Isolation and characterization of the Schizosaccharomyces pombe rhp9 gene: a gene required for the DNA damage checkpoint but not the replication checkpoint
Department of Biochemistry, School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK.
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