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Nucleic Acids Research, Vol 25, Issue 11 2229-2230, Copyright © 1997 by Oxford University Press

ARTICLES

Simultaneous loading of 200 sample lanes for DNA sequencing on vertical and horizontal, standard and ultrathin gels

H Erfle, R Ventzki, H Voss, S Rechmann, V Benes, J Stegemann and W Ansorge
Biochemical Instrumentation, EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany.

We have developed a simple and efficient technique for automated parallel loading of >/=200 lanes on a 30 cm-wide gel in automated DNA sequencing, using porous filter materials and an associated manual or robotic system. The samples are loaded onto the teeth of a comb made of the porous material. The comb, with samples, is inserted directly above the straight edge of the polymerized gel. The samples are driven from the comb into the gel by the applied electrical field. A particularly advantageous aspect of this method is the elimination of the thin gel walls separating the sample wells in the standard gel loading technique. The time for sample loading is significantly reduced to a few minutes. The loading technique is applicable to horizontal or vertical systems, with standard or ultrathin gels.
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