Nucleic Acids Research, Vol 25, Issue 12 2311-2318, Copyright © 1997 by Oxford University Press
T Hermann, B Schmid, H Heumann and HU Goringer
RNA editing in protozoan parasites is a mitochondrial RNA processing
reaction in which exclusively uridylate residues are inserted into, and
less frequently deleted from, pre-mRNAs. Molecules central to the process
are so-called guide RNAs (gRNAs) which function as templates in the
reaction. For a detailed molecular understanding of the mechanism of the
editing process knowledge of structural features of gRNAs will be
essential. Here we report on a computer-assisted molecular modelling
approach to construct the first three-dimensional gRNA model for gND7- 506,
a ND7-specific gRNA from Trypanosoma brucei. The modelling process relied
on chemical modification and enzymatic probing data and was validated by in
vitro mutagenesis experiments. The model predicts a reasonably compact
structure, where two stem/loop secondary structure elements are brought
into close proximity by a triple A tertiary interaction, forming a core
element within the centre of the molecule. The model further suggests that
the surface of the gRNA is primarily made up of the sugar-phoshate
backbone. On the basis of the model, footprinting experiments of gND7-506
in a complex with the gRNA binding protein gBP21 could successfully be
interpreted and provide a first picture for the assembly of gRNAs within a
ribonucleoprotein complex.
ARTICLES
A three-dimensional working model for a guide RNA from Trypanosoma brucei
Laboratorium fur Molekulare Biologie, Genzentrum der Ludwig Maximillians Universitat Munchen, 82152 Martinsried, Germany.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Reifur and D. J. Koslowsky Trypanosoma brucei ATPase subunit 6 mRNA bound to gA6-14 forms a conserved three-helical structure RNA, October 1, 2008; 14(10): 2195 - 2211. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Madej, J. D. Alfonzo, and A. Huttenhofer Small ncRNA transcriptome analysis from kinetoplast mitochondria of Leishmania tarentolae Nucleic Acids Res., March 12, 2007; 35(5): 1544 - 1554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. GOLDEN and S. L. HAJDUK The 3'-untranslated region of cytochrome oxidase II mRNA functions in RNA editing of African trypanosomes exclusively as a cis guide RNA RNA, January 1, 2005; 11(1): 29 - 37. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. SIMPSON, R. APHASIZHEV, G. GAO, and X. KANG Mitochondrial proteins and complexes in Leishmania and Trypanosoma involved in U-insertion/deletion RNA editing RNA, February 1, 2004; 10(2): 159 - 170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. SIMPSON, S. SBICEGO, and R. APHASIZHEV Uridine insertion/deletion RNA editing in trypanosome mitochondria: A complex business RNA, March 1, 2003; 9(3): 265 - 276. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lorger, M. Engstler, M. Homann, and H. U. Goringer Targeting the Variable Surface of African Trypanosomes with Variant Surface Glycoprotein-Specific, Serum-Stable RNA Aptamers Eukaryot. Cell, February 1, 2003; 2(1): 84 - 94. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. F. Muller and H. U. Goringer Mechanism of the gBP21-mediated RNA/RNA annealing reaction: matchmaking and charge reduction Nucleic Acids Res., January 15, 2002; 30(2): 447 - 455. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Cruz-Reyes, A. Zhelonkina, L. Rusche, and B. Sollner-Webb Trypanosome RNA Editing: Simple Guide RNA Features Enhance U Deletion 100-Fold Mol. Cell. Biol., February 1, 2001; 21(3): 884 - 892. [Abstract] [Full Text]
|
|
|
|
|
|
S. S. Leung and D. J. Koslowsky RNA editing in Trypanosoma brucei: characterization of gRNA U-tail interactions with partially edited mRNA substrates Nucleic Acids Res., February 1, 2001; 29(3): 703 - 709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pelletier, M. M. Miller, and L. K. Read RNA-binding properties of the mitochondrial Y-box protein RBP16 Nucleic Acids Res., March 1, 2000; 28(5): 1266 - 1275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Hayman and L. K. Read Trypanosoma brucei RBP16 Is a Mitochondrial Y-box Family Protein with Guide RNA Binding Activity J. Biol. Chem., April 23, 1999; 274(17): 12067 - 12074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Allen, S. Heidmann, R. Reed, P. J. Myler, H. U. Göringer, and K. D. Stuart Association of Guide RNA Binding Protein gBP21 with Active RNA Editing Complexes in Trypanosoma brucei Mol. Cell. Biol., October 1, 1998; 18(10): 6014 - 6022. [Abstract] [Full Text]
|
|