Nucleic Acids Research, Vol 25, Issue 12 2344-2351, Copyright © 1997 by Oxford University Press
C Phillips and A Virtanen
Regulation of polyadenylation efficiency at the secretory poly(A) site
plays an essential role in gene expression at the immunoglobulin (IgM)
locus. At this poly(A) site the consensus AAUAAA hexanucleotide sequence is
embedded in an extended AU-rich region and there are two downstream GU-rich
regions which are suboptimally placed. As these sequences are involved in
formation of the polyadenylation pre- initiation complex, we examined their
function in vivo and in vitro . We show that the upstream AU-rich region
can function in the absence of the consensus hexanucleotide sequence both
in vivo and in vitro and that both GU-rich regions are necessary for full
polyadenylation activity in vivo and for formation of
polyadenylation-specific complexes in vitro . Sequence comparisons reveal
that: (i) the dual structure is distinct for the IgM secretory poly(A) site
compared with other immunoglobulin isotype secretory poly(A) sites; (ii)
the presence of an AU-rich region close to the consensus hexanucleotide is
evolutionarily conserved for IgM secretory poly(A) sites. We propose that
the dual structure of the IgM secretory poly(A) site provides a flexibility
to accommodate changes in polyadenylation complex components during
regulation of polyadenylation efficiency.
ARTICLES
The murine IgM secretory poly(A) site contains dual upstream and downstream elements which affect polyadenylation
Department of Medical Genetics, Uppsala University, Biomedical Centre, Box 589, SE-751 23 Uppsala, Sweden.
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