Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (41K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Wright, D. A.
Right arrow Articles by Voytas, D. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wright, D. A.
Right arrow Articles by Voytas, D. F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 25, Issue 13 2679-2680, Copyright © 1997 by Oxford University Press

ARTICLES

Recovery of YAC-end sequences through complementation of an Escherichia coli pyrF mutation

DA Wright, SK Park, D Wu, GJ Phillips, SR Rodermel and DF Voytas
Department of Zoology and Genetics, Iowa State University, Ames, IA 50011, USA.

We have developed a genetic means to recover sequences from YAC-ends near the yeast selectable marker URA3. This strategy is based on the ability of URA3 to complement mutations in pyrF, an Escherichia coli gene required for pyrimidine biosynthesis. We have developed an E.coli strain with a non-reverting allele of pyrF that is also suitable for cloning (recA-, hsdR-). We demonstrate the utility of this complementation strategy to obtain right-end clones from three YACs containing Arabidopsis thaliana DNA.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
N. Wu and E. F. Pai
Crystal Structures of Inhibitor Complexes Reveal an Alternate Binding Mode in Orotidine-5'-monophosphate Decarboxylase
J. Biol. Chem., July 26, 2002; 277(31): 28080 - 28087.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. Wu, D. A. Wright, C. Wetzel, D. F. Voytas, and S. Rodermel
The IMMUTANS Variegation Locus of Arabidopsis Defines a Mitochondrial Alternative Oxidase Homolog That Functions during Early Chloroplast Biogenesis
PLANT CELL, January 1, 1999; 11(1): 43 - 56.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.