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Nucleic Acids Research, Vol 25, Issue 14 2943-2944, Copyright © 1997 by Oxford University Press

ARTICLES

Avoidance of sulfur loss during ammonia treatment of oligonucleotide phosphorothioates

CB Reese and Q Song
Department of Chemistry, King's College London, Strand, London WC2R 2LS, UK.

Sulfur loss during the unblocking of phosphorothioate analogues of oligonucleotides with concentrated aqueous ammonia can be completely suppressed by the addition of 2-mercaptoethanol.
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