Nucleic Acids Research, Vol 25, Issue 17 3379-3388, Copyright © 1997 by Oxford University Press
M Belfort and RJ Roberts
Homing endonucleases are rare-cutting enzymes encoded by introns and
inteins. They have striking structural and functional properties that
distinguish them from restriction enzymes. Nomenclature conventions
analogous to those for restriction enzymes have been developed for the
homing endonucleases. Recent progress in understanding the structure and
function of the four families of homing enzymes is reviewed. Of particular
interest are the first reported structures of homing endonucleases of the
LAGLIDADG family. The exploitation of the homing enzymes in genome analysis
and recombination research is also summarized. Finally, the evolution of
homing endonucleases is considered, both at the structure-function level
and in terms of their persistence in widely divergent biological systems.
REVIEWS
Homing endonucleases: keeping the house in order
Molecular Genetics Program, Wadsworth Center, New York State Department of Health, and School of Public Health, State University of New York at Albany, PO Box 22002, Albany, New York 12201-2002, USA. marlene.belfort@wadsworth.org
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M. R. Goddard and A. Burt Recurrent invasion and extinction of a selfish gene PNAS, November 23, 1999; 96(24): 13880 - 13885. [Abstract] [Full Text] [PDF]
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A. J. Pommer, U. C. Kuhlmann, A. Cooper, A. M. Hemmings, G. R. Moore, R. James, and C. Kleanthous Homing in on the Role of Transition Metals in the HNH Motif of Colicin Endonucleases J. Biol. Chem., September 17, 1999; 274(38): 27153 - 27160. [Abstract] [Full Text] [PDF]
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H. Mizumura, T. Shibata, and N. Morishima Stable Association of 70-kDa Heat Shock Protein Induces Latent Multisite Specificity of a Unisite-specific Endonuclease in Yeast Mitochondria J. Biol. Chem., September 3, 1999; 274(36): 25682 - 25690. [Abstract] [Full Text] [PDF]
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K. D. E. Everett, S. Kahane, R. M. Bush, and M. G. Friedman An Unspliced Group I Intron in 23S rRNA Links Chlamydiales, Chloroplasts, and Mitochondria J. Bacteriol., August 15, 1999; 181(16): 4734 - 4740. [Abstract] [Full Text]
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L. K. Lewis, J. W. Westmoreland, and M. A. Resnick Repair of Endonuclease-Induced Double-Strand Breaks in Saccharomyces cerevisiae: Essential Role for Genes Associated with Nonhomologous End-Joining Genetics, August 1, 1999; 152(4): 1513 - 1529. [Abstract] [Full Text]
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V. Pingoud, H. Thole, F. Christ, W. Grindl, W. Wende, and A. Pingoud Photocross-linking of the Homing Endonuclease PI-SceI to Its Recognition Sequence J. Biol. Chem., April 9, 1999; 274(15): 10235 - 10243. [Abstract] [Full Text] [PDF]
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F. S. Gimble, X. Duan, D. Hu, and F. A. Quiocho Identification of Lys-403 in the PI-SceI Homing Endonuclease as Part of a Symmetric Catalytic Center J. Biol. Chem., November 13, 1998; 273(46): 30524 - 30529. [Abstract] [Full Text] [PDF]
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T. P. Gary, N. E. Colowick, and G. Mosig A Species Barrier Between Bacteriophages T2 and T4: Exclusion, Join-Copy and Join-Cut-Copy Recombination and Mutagenesis in the dCTPase Genes Genetics, April 1, 1998; 148(4): 1461 - 1473. [Abstract] [Full Text] [PDF]
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V. Derbyshire, D. W. Wood, W. Wu, J. T. Dansereau, J. Z. Dalgaard, and M. Belfort Genetic definition of a protein-splicing domain: Functional mini-inteins support structure predictions and a model for intein evolution PNAS, October 14, 1997; 94(21): 11466 - 11471. [Abstract] [Full Text] [PDF]
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L. Chen, J. Benner, and F. B. Perler Protein Splicing in the Absence of an Intein Penultimate Histidine J. Biol. Chem., June 30, 2000; 275(27): 20431 - 20435. [Abstract] [Full Text] [PDF]
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B. Beylot and A. Spassky Chemical Probing Shows That the Intron-encoded Endonuclease I-SceI Distorts DNA through Binding in Monomeric Form to Its Homing Site J. Biol. Chem., June 29, 2001; 276(27): 25243 - 25253. [Abstract] [Full Text] [PDF]
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Q. Xu, S. Stickel, R. J. Roberts, M. J. Blaser, and R. D. Morgan Purification of the Novel Endonuclease, Hpy188I, and Cloning of Its Restriction-Modification Genes Reveal Evidence of Its Horizontal Transfer to the Helicobacter pylori Genome J. Biol. Chem., May 26, 2000; 275(22): 17086 - 17093. [Abstract] [Full Text] [PDF]
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