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Nucleic Acids Research, Vol 25, Issue 18 3665-3671, Copyright © 1997 by Oxford University Press

ARTICLES

FLP-mediated DNA mobilization to specific target sites in Drosophila chromosomes

MM Golic, YS Rong, RB Petersen, SL Lindquist and KG Golic
Department of Biology, University of Utah, Salt Lake City, UT 84112, USA. golic@bioscience.utah.edu

The ability to place a series of gene constructs at a specific site in the genome opens new possibilities for the experimental examination of gene expression and chromosomal position effects. We report that the FLP- FRT site-specific recombination system of the yeast 2mu plasmid can be used to integrate DNA at a chromosomal FRT target site in Drosophila. The technique we used was to first integrate an FRT- flanked gene by standard P element-mediated transformation. FLP was then used to excise the FRT- flanked donor DNA and screen for FLP- mediated re-integration at an FRT target at a different chromosome location. Such events were recovered from up to 5% of the crosses used to screen for mobilization and are easily detectable by altered linkage of a white reporter gene or by the generation of a white + gene upon integration.
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