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Nucleic Acids Research, Vol 25, Issue 18 3743-3744, Copyright © 1997 by Oxford University Press

ARTICLES

Use of the two-hybrid system to identify protein-protein interaction temperature-sensitive mutants: application to the CDK2/p21Cip1 interaction

C Cayrol, G Cabrolier and B Ducommun
Institut de Pharmacologie et de Biologie Structurale du CNRS, UPR 9062, Universite Paul Sabatier, 205 route de Narbonne, 31077 Toulouse, France.

We describe the application of the two-hybrid system to the identification of protein-protein interaction temperature-sensitive mutants. We applied this strategy to the interaction between the human CDK2 cell cycle regulator and the p21Cip1 regulatory subunit. A library of randomly generated CDK2 mutant proteins was screened for interaction with p21Cip1 at different temperatures. This approach resulted in the isolation of single point mutations in CDK2 causing temperature- sensitive interaction with p21Cip1. Our results demonstrate that the two-temperature two-hybrid screen is an efficient approach for the rational design and screening of protein-protein interaction conditional mutations.
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