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Nucleic Acids Research, Vol 25, Issue 18 3747-3748, Copyright © 1997 by Oxford University Press

ARTICLES

Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single- stranded DNA/RNA using T4 RNA ligase

Y Kinoshita, K Nishigaki and Y Husimi
Department of Functional Materials Science, Saitama University, 255 Shimo-okubo, Urawa 338, Japan.

A rapid 5'-labeling method of single-stranded DNA/RNA was developed, which is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5'-ends of both oligo- and polynucleotides.
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