Nucleic Acids Research, Vol 25, Issue 21 4323-4330, Copyright © 1997 by Oxford University Press
KU Wagner, RJ Wall, L St-Onge, P Gruss, A Wynshaw-Boris, L Garrett, M Li, PA Furth and L Hennighausen
To delete genes specifically from mammary tissue using the Cre-lox system,
we have established transgenic mice expressing Cre recombinase under
control of the WAP gene promoter and the MMTV LTR. Cre activity in these
mice was evaluated by three criteria. First, the tissue distribution of Cre
mRNA was analyzed. Second, an adenovirus carrying a reporter gene was used
to determine expression at the level of single cells. Third, tissue
specificity of Cre activity was determined in a mouse strain carrying a
reporter gene. In adult MMTV-Cre mice expression of the transgene was
confined to striated ductal cells of the salivary gland and mammary
epithelial cells in virgin and lactating mice. Expression of WAP-Cre was
only detected in alveolar epithelial cells of mammary tissue during
lactation. Analysis of transgenic mice carrying both the MMTV-Cre and the
reporter transgenes revealed recombination in every tissue. In contrast,
recombination mediated by Cre under control of the WAP gene promoter was
largely restricted to the mammary gland but occasionally observed in the
brain. These results show that transgenic mice with WAP-Cre but not
MMTV-Cre can be used as a powerful tool to study gene function in
development and tumorigenesis in the mammary gland.
ARTICLES
Cre-mediated gene deletion in the mammary gland
Laboratory of Metabolism and Biochemistry, National Institute of Diabetes Digestive and Kidney Diseases and Laboratory of Genetic Disease Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA,
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