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Nucleic Acids Research, Vol 25, Issue 22 4608-4613, Copyright © 1997 by Oxford University Press

ARTICLES

Opening of the extraordinarily stable mini-hairpin d(GCGAAGC)

B Jolles, M Refregiers and A Laigle
LPBC (CNRS URA 2056), Universite Pierre et Marie Curie, 4 Place Jussieu, Case 138, 75252 Paris Cedex 05, France. jolles@lpbc.jussieu.fr

For the purposes of the antisense strategy oligodeoxyribonucleotides can be protected against serum and cell nuclease digestion by tagging at their 3'-end with a sequence naturally forming a very stable hairpin, d(GCGAAGC). This nuclease-resistant hairpin is also known for its high thermostability. We demonstrate in this study that attachment of d(GCGAAGC) at the 3'-end of an oligodeoxyribonucleotide does not hinder hybridization of the 5'-part of this oligonucleotide to a complementary DNA strand. Moreover, the hairpin is in equilibrium between a folded and an open structure, with an energy minimum in favor of pairing if it is possible, even with mismatches.
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