Nucleic Acids Research, Vol 25, Issue 22 4639-4643, Copyright © 1997 by Oxford University Press
G Haaima, HF Hansen, L Christensen, O Dahl and PE Nielsen
The synthesis of a diaminopurine PNA monomer, N-[N6-(benzyloxycarbonyl)-
2,6-diaminopurine-9-yl] acetyl-N-(2-t- butyloxycarbonylaminoethyl)glycine,
and the incorporation of this monomer into PNA oligomers are described.
Substitution of adenine by diaminopurine in PNA oligomers increased the T m
of duplexes formed with complementary DNA, RNA or PNA by 2.5-6.5 degrees C
per diaminopurine. Furthermore, discrimination against mismatches facing
the diaminopurine in the hybridizing oligomer is improved. Finally, a
homopurine decamer PNA containing six diaminopurines is shown to form a
(gel shift) stable strand displacement complex with a target in a 246 bp
double-stranded DNA fragment.
ARTICLES
Increased DNA binding and sequence discrimination of PNA oligomers containing 2,6-diaminopurine
Center for Biomolecular Recognition, Department of Chemistry, The H. C.Orsted Institute, Universitetsparken 5, DK-2100 Copenhagen O, Denmark.
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