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Nucleic Acids Research, Vol 25, Issue 22 4690-4691, Copyright © 1997 by Oxford University Press

ARTICLES

Construction of a semisynthetic antibody library using trinucleotide oligos

M Braunagel and M Little
Recombinant Antibody Group, Division of Diagnosis and Experimental Therapy, Deutsches Krebsforschungszentrum, INF 280, 69120 Heidelberg, Germany. affitech@online.no

A semisynthetic antibody library composed of single chain Fv fragments (scFv) was constructed by replacing the heavy chain CDR3 region of a human scFv by a random sequence of eight amino acids using trinucleotide codons. After cloning into a phage display vector, an antibody library was generated with a complexity of 8 x 10(8) independent clones. The library was screened for binders to dinitrophenol, fluorescein isothiocyanate and 3-nitro-4-hydroxy-5- iodophenylacetic acid. scFv antibodies that specifically bound the antigen were obtained in each case.
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