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Nucleic Acids Research, Vol 25, Issue 22 4697-4699, Copyright © 1997 by Oxford University Press

ARTICLES

Monitoring the amplification of CATCH, a 3SR based cooperatively coupled isothermal amplification system, by fluorimetric methods

R Ehricht, T Kirner, T Ellinger, P Foerster and JS McCaskill
Department of Molecular Information Processing, Institute of Molecular Biotechnology, Beutenbergstrasse 11, 07745 Jena, Germany. rehr@imb- jena.de

Three different types of fluorescence detection methods were employed to monitor amplification of a previously established isothermal cooperatively coupled amplification system as it can serve as a tool for the investigation of fundamental issues in evolutionary optimization. By using 5'IRD-41 fluorescent labeled primers, the intercalating dye TOPRO-1 and a 5'fluorescin/3'DABCYL 4-(4- dimethylamino-phenylazo)benzoic acid labeled ss 24 nt DNA, evolving molecular cooperation is accessible, sequence specifically as well as non-sequence-specifically without using radioactivity.
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