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Nucleic Acids Research, Vol 25, Issue 24 5125-5126, Copyright © 1997 by Oxford University Press

ARTICLES

Detection of a single base exchange in PCR-amplified DNA fragments using agarose gel electrophoresis containing bisbenzimide-PEG

M Muller, L Kruse, AM Tabrett and DJ Barbara
Hanse Analytik GmbH, Fahrenheitstrasse 1, D-28359 Bremen, Germany. m.mueller@hanse-analytik.de

Using PCR fragments of known sequences derived from isolates of two related fungal species, simple submarine electrophoresis in agarose gels containing a bisbenzimide-PEG conjugate (H.A.-Yellow) has been shown to be capable of distinguishing DNA fragments 567 bp long which differ by as little as a single base change. However, only changes affecting bisbenzimide binding sites (which consist of at least four consecutive A/T bases) alter mobility; other changes are ineffective. A second ligand (H.A.-Red) with high G/C specificity is suggested which may be as effective in detecting other sequence changes.
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 Proc. Natl. Acad. Sci. USAHome page
G. L. Igloi
Variability in the stability of DNA-peptide nucleic acid (PNA) single-base mismatched duplexes: Real-time hybridization during affinity electrophoresis in PNA-containing gels
PNAS, July 21, 1998; 95(15): 8562 - 8567.
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