Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (109K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Dioh, W.
Right arrow Articles by Lebrun, M. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dioh, W.
Right arrow Articles by Lebrun, M. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 25, Issue 24 5130-5131, Copyright © 1997 by Oxford University Press

ARTICLES

RAPD-based screening of genomic libraries for positional cloning

W Dioh, D Tharreau and MH Lebrun
Genetique Moleculaire des Champignons Phytopathogenes, Institut de Genetique et Microbiologie, CNRS-URA 2255, Batiment 400, Universite Paris-Sud, 91405 Orsay, France.

RAPD markers are frequently used for positional cloning. However, RAPD markers often contain repeated sequences which prevent genomic library screening by hybridisation. We have developed a simple RAPD analysis of genomic libraries based on the identification of cosmid pools and clones amplifying the RAPD marker of interest. Our method does not require the cloning or characterisation of the RAPD marker as it relies on the analysis of cosmid pools or clones using a simple RAPD protocol. We applied this strategy using four RAPD markers composed of single copy or repeated sequences linked to avirulence genes of the rice blast fungus Magnaporthe grisea . Cosmids containing these RAPD markers were easily and rapidly identified allowing the construction of physical contigs at these loci.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
H. U. Bohnert, I. Fudal, W. Dioh, D. Tharreau, J.-L. Notteghem, and M.-H. Lebrun
A Putative Polyketide Synthase/Peptide Synthetase from Magnaporthe grisea Signals Pathogen Attack to Resistant Rice
PLANT CELL, September 1, 2004; 16(9): 2499 - 2513.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Xu, D. Yang, J. Domingo, J. Ni, and N. Huang
Screening for overlapping bacterial artificial chromosome clones by PCR analysis with an arbitrary primer
PNAS, May 12, 1998; 95(10): 5661 - 5666.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.