Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (226K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Backert, S.
Right arrow Articles by Borner, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Backert, S.
Right arrow Articles by Borner, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 25, Issue 3 582-589, Copyright © 1997 by Oxford University Press

ARTICLES

Unique features of the mitochondrial rolling circle-plasmid mp1 from the higher plant Chenopodium album (L.)

S Backert, K Meissner and T Borner
Institut fur Biologie, Humboldt-Universitat zu Berlin, Chausseestrasse 117, D-10115 Berlin, Germany.

We analyzed the structure and replication of the mitochondrial (mt) circular DNA plasmid mp1 (1309 bp) from the higher plant Chenopodium album(L.). Two dimensional gel electrophoresis (2DE) revealed the existence of oligomers of up to a decamer in addition to the prevailing monomeric form. The migration behavior of cut replication intermediates during 2DE was consistent with a rolling circle (RC) type of replication. We detected entirely single-stranded (ss) plasmid copies hybridizing only with one of the two DNA strands. This result indicates the occurence of an asymmetric RC replication mechanism. mp1 has, with respect to its replication, some unique features compared with bacterial RC plasmids. We identified and localized a strand-specific nicking site (origin of RC replication) on the plasmid by primer extension studies. Nicks in the plasmid were found to occur at any one of six nucleotides (TAAG/GG) around position 735 of the leading strand. This sequence shows no homology to origin motifs from known bacterial RC replicons. mp1 is the first described RC plasmid in a higher plant.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 MicrobiologyHome page
S. Backert, T. Kwok, and W. Konig
Conjugative plasmid DNA transfer in Helicobacter pylori mediated by chromosomally encoded relaxase and TraG-like proteins
Microbiology, November 1, 2005; 151(11): 3493 - 3503.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Mackenzie and L. McIntosh
Higher Plant Mitochondria
PLANT CELL, April 1, 1999; 11(4): 571 - 586.
[Full Text]

Home page
 Plant CellHome page
H. Janska, R. Sarria, M. Woloszynska, M. Arrieta-Montiel, and S. A. Mackenzie
Stoichiometric Shifts in the Common Bean Mitochondrial Genome Leading to Male Sterility and Spontaneous Reversion to Fertility
PLANT CELL, July 1, 1998; 10(7): 1163 - 1180.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.