Nucleic Acids Research, Vol 25, Issue 3 641-647, Copyright © 1997 by Oxford University Press
AJ Bobb, MS Chern and MM Bustos
Transcription of genes DLEC2 and PHSbeta is specifically and coordinately
regulated during maturation of Phaseolus embryos. Over- expression of the
seed- specific factor PvALF in cotyledon cells results in transactivation
of either promoter. PvALF is related to the VP1 protein of maize, which
transactivates gene expression via G-boxes, Sph elements and AT-rich
sequences. We used deletions and base substitutions in the DLEC2 and
PHSbeta promoters to demonstrate that several conserved RY-repeats were
necessary for PvALF induction of both genes. A comprehensive mutational and
transactivation analysis was used to define functionally the sequence of
the DLEC2 repeat RY3 asG/CCATGCxxG/C. We also found that an interaction
between RY3 and the 3'-flanking tetranucleotide CCAC increased PvALF
transactivation. A preferred spacing and phasing requirement for the RY3
and CCAC motifs suggested the possibility of interactions between cellular
factors that recognize either element. The high conservation of Sph-RY
motifs in seed-specific promoters from monocots and dicots indicates that
organ and temporal specification by factors similar to VP1 and PvALF is
common among seed plants.
ARTICLES
Conserved RY-repeats mediate transactivation of seed-specific promoters by the developmental regulator PvALF
Department of Biological Sciences, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA.
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