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Nucleic Acids Research, Vol 25, Issue 6 1309-1310, Copyright © 1997 by Oxford University Press

ARTICLES

A simple procedure for constructing 5'-amino-terminated oligodeoxynucleotides in aqueous solution

RK Bruick, M Koppitz, GF Joyce and LE Orgel
Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.

A rapid method for the synthesis of oligodeoxynucleotides (ODNs) terminated by 5'-amino-5'-deoxythymidine is described. A 3'- phosphorylated ODN (the donor) is incubated in aqueous solution with 5'- amino- 5'-deoxythymidine in the presence of N-(3-dimethylaminopropyl)- N'-ethylcarbodiimide hydrochloride (EDC), extending the donor by one residue via a phosphoramidate bond. Template- directed ligation of the extended donor and an acceptor ODN, followed by acid hydrolysis, yields the acceptor ODN extended by a single 5'-amino-5'-deoxythymidine residue at its 5'terminus.
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