Nucleic Acids Research, Vol 25, Issue 8 1578-1584, Copyright © 1997 by Oxford University Press
H Takahashi, S Okazaki and H Fujiwara
The telomeres of the silkworm, Bombyx mori, consist of pentanucleotide
repeats (TTAGG)n . We previously characterized the non-LTR element TRAS1,
which terminates with oligo (A) in a head to tail orientation at the exact
position (between A and C) of the (CCTAA) n repeats. Here we characterized
another family of telomere-specific non-LTR retrotransposon named SART1.
The SART1 family was inserted at another site of the (TTAGG) n in a reverse
orientation from that of TRAS1. The complete unit of SART1, 6.7 kb in
length with a poly (A) stretch, contains two open reading frames encoding
putative gag and pol products, overlapping by 54 bp in the -1 reading
frame. Most of the 600 SART1 copies in the silkworm haploid genome are
completely conserved in structure without 5'truncation. All SART1 sequences
analyzed were inserted at the same position (between T and A) within the
(TTAGG) n repeats. Fluorescence in situ hybridization showed that many of
the SART1 copies were localized in the chromosomal ends. A phylogenetic
tree showed that the SART1, TRAS1 and two other site-specific elements, R1
and RT, which insert into 28S ribosomal RNA genes in insects, belong to the
same group. Based on the orientation for the chromosomal insertion and
structural similarities, these elements could be further classified into
two subgroups, R1/TRAS1 and RT/SART1, suggesting that the target
specificity of the two telomere-associated elements was changed
independently.
ARTICLES
A new family of site-specific retrotransposons, SART1, is inserted into telomeric repeats of the silkworm, Bombyx mori
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan.
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