Nucleic Acids Research, Vol 25, Issue 8 1641-1648, Copyright © 1997 by Oxford University Press
MR Shen, J Brosius and PL Deininger
ID elements are short interspersed elements (SINEs) found in high copy
number in many rodent genomes. BC1 RNA, an ID-related transcript, is
derived from the single copy BC1 RNA gene. The BC1 RNA gene has been shown
to be a master gene for ID element amplification in rodent genomes. ID
elements are dispersed through a process termed retroposition. The
retroposition process involves a number of potential regulatory steps.
These regulatory steps may include transcription in the appropriate tissue,
transcript stability, priming of the RNA transcript for reverse
transcription and integration. This study focuses on priming of the RNA
transcript for reverse transcription. BC1 RNA gene transcripts are shown to
be able to prime their own reverse transcription in an efficient
intramolecular and site-specific fashion. This self-priming ability is a
consequence of the secondary structure of the 3'-unique region. The
observation that a gene actively amplified throughout rodent evolution
makes a RNA capable of efficient self- primed reverse transcription
strongly suggests that self-priming is at least one feature establishing
the BC1 RNA gene as a master gene for amplification of ID elements.
ARTICLES
BC1 RNA, the transcript from a master gene for ID element amplification, is able to prime its own reverse transcription
Department of Biochemistry and Molecular Biology, Neuroscience Center of Excellence, Stanley S.Scott Cancer Center, Louisiana State University Medical Center, 1901 Perdido Street, New Orleans, LA 70112, USA. shen3@llnl.gov
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