Nucleic Acids Research, Vol 26, Issue 10 2420-2425, Copyright © 1998 by Oxford University Press
K Kimura, H Saga, K Hayashi, H Obata, Y Chimori, H Ariga and K Sobue
The expression of alpha-smooth muscle actin is coordinately regulated by
positive and negative cis- elements in the promoter region. Although cis
-elements and trans -acting factors involved in the positive regulation of
the alpha-smooth muscle (alpha-SM) actin gene have been well characterized,
details of negative regulation remain unclear. In functional analyses using
cultured gizzard smooth muscle cells, we identified a sequence ranging from
-238 to -219 in the promoter region as a novel negative element. Mutation
and deletion analyses further revealed that a sequence, TATCTTA (-228 to
-222), is essential for negative regulation. Gel shift assay and
Southwestern blotting indicated that a nuclear protein factor specifically
interacts with single- or double-strand DNA including this sequence, and
the protein factor displays a highly potent binding to the sense strand
DNA. cDNA cloning and gel shift analysis using anti-MSSP-1 antibodies
revealed that this protein factor is a chicken homolog of human MSSP-1 (c-
myc gene single-strand binding protein-1). In fact, overexpression of MSSP-
1 in cultured smooth muscle cells suppresses the promoter activity. These
results suggest a novel function of MSSP-1 regarding the transcriptional
regulation of alpha-sm actin gene.
ARTICLES
c-Myc gene single-strand binding protein-1, MSSP-1, suppresses transcription of alpha-smooth muscle actin gene in chicken visceral smooth muscle cells
Department of Neurochemistry and Neuropharmacology, Biomedical Research Center, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565, Japan.
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