Nucleic Acids Research, Vol 26, Issue 11 2565-2572, Copyright © 1998 by Oxford University Press
M Nashimoto, S Geary, M Tamura and R Kaspar
Mammalian tRNA 3' processing endoribonuclease (3' tRNase) can recognize and
cleave any target RNA that forms a precursor tRNA-like complex with another
RNA. Various sets of RNA molecules were tested to identify the smallest RNA
that can direct target RNA cleavage by 3' tRNase. A 3' half tRNAArgwas
cleaved efficiently by 3' tRNase in the presence of small 5' half
tRNAArgvariants, the D stem-loop region of which was partially deleted.
Remarkably, 3' tRNase also cleaved the 3' half tRNAArgin the presence of a
7 nt 5' tRNAArg composed only of the acceptor stem region with a catalytic
efficiency comparable with that of cleavage directed by an intact 5' half
tRNAArg. The catalytic efficiency of cleavage directed by the heptamer
decreased as the stability of the T stem-loop structures of 3' half tRNAArg
variants decreased. No heptamer-directed cleavage of a 3' half tRNAArg
without T stem base pairs was detected. A heptamer also directed cleavage
of an HIV-1 RNA containing a stable hairpin structure. These findings
suggest that in the presence of an RNA heptamer, 3' tRNase can discriminate
and eliminate target RNAs that possess a stable hairpin adjacent to the
heptamer binding sequence from a large complex RNA pool.
ARTICLES
RNA heptamers that direct RNA cleavage by mammalian tRNA 3' processing endoribonuclease
Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA. mnashimoto@chemgate.byu.edu
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. J. Scherer, R. Frank, and J. J. Rossi Optimization and characterization of tRNA-shRNA expression constructs Nucleic Acids Res., April 10, 2007; (2007) gkm103v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. S. Shibata, H. Takaku, M. Takagi, and M. Nashimoto The T Loop Structure Is Dispensable for Substrate Recognition by tRNase ZL J. Biol. Chem., June 10, 2005; 280(23): 22326 - 22334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Habu, N. Miyano-Kurosaki, M. Kitano, Y. Endo, M. Yukita, S. Ohira, H. Takaku, M. Nashimoto, and H. Takaku Inhibition of HIV-1 gene expression by retroviral vector-mediated small-guide RNAs that direct specific RNA cleavage by tRNase ZL Nucleic Acids Res., January 12, 2005; 33(1): 235 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Takaku, A. Minagawa, M. Takagi, and M. Nashimoto The N-terminal half-domain of the long form of tRNase Z is required for the RNase 65 activity Nucleic Acids Res., August 18, 2004; 32(15): 4429 - 4438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Takaku, A. Minagawa, M. Takagi, and M. Nashimoto A novel 4-base-recognizing RNA cutter that can remove the single 3' terminal nucleotides from RNA molecules Nucleic Acids Res., June 28, 2004; 32(11): e91 - e91. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tamura, C. Nashimoto, N. Miyake, Y. Daikuhara, K. Ochi, and M. Nashimoto Intracellular mRNA cleavage by 3' tRNase under the direction of 2'-O-methyl RNA heptamers Nucleic Acids Res., August 1, 2003; 31(15): 4354 - 4360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Takaku, A. Minagawa, M. Takagi, and M. Nashimoto A candidate prostate cancer susceptibility gene encodes tRNA 3' processing endoribonuclease Nucleic Acids Res., May 1, 2003; 31(9): 2272 - 2278. [Abstract] [Full Text] [PDF]
|
|