Nucleic Acids Research, Vol 26, Issue 12 3013-3017, Copyright © 1998 by Oxford University Press
GJ Schouten, HG van Luenen, NC Verra, D Valerio and RH Plasterk
The transposon Tc1 of the nematode Caenorhabditis elegans is a member of
the widespread family of Tc1/mariner transposons. The distribution pattern
of virtually identical transposons among insect species that diverged 200
million years ago suggested horizontal transfer of the elements between
species. Thishypothesis gained experimental support when it was shown that
Tc1 and later also mariner transposons could be made to jump in vitro ,
with their transposase as the only protein required. Later it was shown
that mariner transposons from one fruit fly species can jump in other fruit
fly species and in a protozoan and, recently, that a Tc1-like transposon
from the nematode jumps in fish cells and that a fish Tc1-like transposon
jumps in human cells. Here we show that the Tc1 element from the nematode
jumps in human cells. This provides further support for the horizontal
spread hypothesis. Furthermore, it suggests that Tc1 can be used as vehicle
for DNA integration in human gene therapy.
ARTICLES
Transposon Tc1 of the nematode Caenorhabditis elegans jumps in human cells
The Netherlands Cancer Institute, Division of Molecular Biology, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.
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