Nucleic Acids Research, Vol 26, Issue 13 3119-3126, Copyright © 1998 by Oxford University Press
R Trippe, B Sandrock and BJ Benecke
HeLa cell extracts contain significant amounts of terminal uridylyl
transferase (TUTase) activity. In a template-independent reaction with
labeled UTP, these enzymes are capable of modifying a broad spectrum of
cellular RNA molecules in vitro . However, fractionation of cell extracts
by gel filtration clearly separated two independent activities. In addition
to a non-specific enzyme, an additional terminal uridylyl transferase has
been identified that is highly specific for cellular and in vitro
synthesized U6 small nuclear RNA (snRNA) molecules. This novel TUTase
enzyme was also able to select as an efficient substrate U6 snRNA species
from higher eucaryotes. In contrast, no labeling was detectable with
purified fission yeast RNA. Using synthetic RNAs containing different
amounts of transcribed 3'-end UMP residues, high resolution gel
electrophoresis revealed that U6 snRNA species with three terminal U
nucleotides served as the optimal substrate for the transferase reaction.
The 3'-end modification of the optimal synthetic substrate was identical to
that observed with endogenous U6 snRNA isolated from HeLa cells. Therefore,
we conclude that the specific addition of UMP residues to 3'-recessed U6
snRNA molecules reflects a recycling process, ensuring the functional
regeneration for pre-mRNA splicing of this snRNA.
ARTICLES
A highly specific terminal uridylyl transferase modifies the 3'-end of U6 small nuclear RNA
Department of Biochemistry NC6, Ruhr University, D-44780 Bochum, Germany.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. B. Patel and M. Bellini The assembly of a spliceosomal small nuclear ribonucleoprotein particle Nucleic Acids Res., November 1, 2008; 36(20): 6482 - 6493. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Licht, J. Medenbach, R. Luhrmann, C. Kambach, and A. Bindereif 3'-cyclic phosphorylation of U6 snRNA leads to recruitment of recycling factor p110 through LSm proteins RNA, August 1, 2008; 14(8): 1532 - 1538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. S. Rissland, A. Mikulasova, and C. J. Norbury Efficient RNA Polyuridylation by Noncanonical Poly(A) Polymerases Mol. Cell. Biol., May 15, 2007; 27(10): 3612 - 3624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Trippe, E. Guschina, M. Hossbach, H. Urlaub, R. Luhrmann, and B.-J. Benecke Identification, cloning, and functional analysis of the human U6 snRNA-specific terminal uridylyl transferase RNA, August 1, 2006; 12(8): 1494 - 1504. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Aphasizheva, R. Aphasizhev, and L. Simpson RNA-editing Terminal Uridylyl Transferase 1: IDENTIFICATION OF FUNCTIONAL DOMAINS BY MUTATIONAL ANALYSIS J. Biol. Chem., June 4, 2004; 279(23): 24123 - 24130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. DUMPELMANN, H. MITTENDORF, and B.-J. BENECKE Efficient transcription of the EBER2 gene depends on the structural integrity of the RNA RNA, April 1, 2003; 9(4): 432 - 442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Aphasizhev and L. Simpson Isolation and Characterization of a U-specific 3'-5'-Exonuclease from Mitochondria of Leishmania tarentolae J. Biol. Chem., June 8, 2001; 276(24): 21280 - 21284. [Abstract] [Full Text] [PDF]
|
|