Nucleic Acids Research, Vol 26, Issue 13 3146-3153, Copyright © 1998 by Oxford University Press
A Errami, DM He, AA Friedl, WJ Overkamp, B Morolli, EA Hendrickson, F Eckardt- Schupp, M Oshimura, PH Lohman, SP Jackson and MZ Zdzienicka
DNA-dependent protein kinase (DNA-PK) plays an important role in DNA
double-strand break (DSB) repair and V(D)J recombination. We have isolated
a new X-ray-sensitive CHO cell line, XR-C1, which is impaired in DSB repair
and which was assigned to complementation group 7, the group that is
defective in the XRCC7 / SCID ( Prkdc ) gene encoding the catalytic subunit
of DNA-PK (DNA-PKcs). Consistent with this complementation analysis, XR-C1
cells lackeddetectable DNA-PKcs protein, did not display DNA-PK catalytic
activity and were complemented by the introduction of a single human
chromosome 8 (providing the Prkdc gene). The impact of the XR-C1 mutation
on V(D)J recombination was quite different from that found in most rodent
cells defective in DNA-PKcs, which are preferentially blocked in coding
joint formation, whereas XR-C1 cells were defective in forming both coding
and signal joints. These results suggest that DNA-PKcs is required for both
coding and signal joint formation during V(D)J recombination and that the
XR-C1 mutant cell line may prove to be a useful tool in understanding this
pathway.
ARTICLES
XR-C1, a new CHO cell mutant which is defective in DNA-PKcs, is impaired in both V(D)J coding and signal joint formation
Department of Radiation Genetics and Chemical Mutagenesis, MGC, Leiden University-Medical Center, Leiden, The Netherlands.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Kuhfittig-Kulle, E. Feldmann, A. Odersky, A. Kuliczkowska, W. Goedecke, A. Eggert, and P. Pfeiffer The mutagenic potential of non-homologous end joining in the absence of the NHEJ core factors Ku70/80, DNA-PKcs and XRCC4-LigIV Mutagenesis, May 1, 2007; 22(3): 217 - 233. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Douglas, X. Cui, W. D. Block, Y. Yu, S. Gupta, Q. Ding, R. Ye, N. Morrice, S. P. Lees-Miller, and K. Meek The DNA-Dependent Protein Kinase Catalytic Subunit Is Phosphorylated In Vivo on Threonine 3950, a Highly Conserved Amino Acid in the Protein Kinase Domain Mol. Cell. Biol., March 1, 2007; 27(5): 1581 - 1591. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.-O. Mari, B. I. Florea, S. P. Persengiev, N. S. Verkaik, H. T. Bruggenwirth, M. Modesti, G. Giglia-Mari, K. Bezstarosti, J. A. A. Demmers, T. M. Luider, et al. Dynamic assembly of end-joining complexes requires interaction between Ku70/80 and XRCC4 PNAS, December 5, 2006; 103(49): 18597 - 18602. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Krawczyk, J. Stap, C. van Oven, R. Hoebe, and J. A. Aten Clustering of double strand break-containing chromosome domains is not inhibited by inactivation of major repair proteins Radiat Prot Dosimetry, December 1, 2006; 122(1-4): 150 - 153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Zhou and L. F. Povirk Extreme cytotoxicity and susceptibility to hprt mutagenesis in Ku-deficient xrs-6 cells treated with bleomycin in plateau phase Mutagenesis, January 1, 2005; 20(1): 39 - 44. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Ding, Y. V. R. Reddy, W. Wang, T. Woods, P. Douglas, D. A. Ramsden, S. P. Lees-Miller, and K. Meek Autophosphorylation of the Catalytic Subunit of the DNA-Dependent Protein Kinase Is Required for Efficient End Processing during DNA Double-Strand Break Repair Mol. Cell. Biol., August 15, 2003; 23(16): 5836 - 5848. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. F. Bender, M. L. Sikes, R. Sullivan, L. E. Huye, M. M. Le Beau, D. B. Roth, O. K. Mirzoeva, E. M. Oltz, and J. H. J. Petrini Cancer predisposition and hematopoietic failure in Rad50S/S mice Genes & Dev., September 1, 2002; 16(17): 2237 - 2251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Fukumura, R. Araki, A. Fujimori, Y. Tsutsumi, A. Kurimasa, G. C. Li, D. J. Chen, K. Tatsumi, and M. Abe Signal Joint Formation Is Also Impaired in DNA-Dependent Protein Kinase Catalytic Subunit Knockout Cells J. Immunol., October 1, 2000; 165(7): 3883 - 3889. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Beamish, R. Jessberger, E. Riballo, A. Priestley, T. Blunt, B. Kysela, and P. A. Jeggo The C-terminal conserved domain of DNA-PKcs, missing in the SCID mouse, is required for kinase activity Nucleic Acids Res., April 1, 2000; 28(7): 1506 - 1513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. K. Shin, T. Rijkers, A. Pastink, and K. Meek Analyses of TCRB Rearrangements Substantiate a Profound Deficit in Recombination Signal Sequence Joining in SCID Foals: Implications for the Role of DNA-Dependent Protein Kinase in V(D)J Recombination J. Immunol., February 1, 2000; 164(3): 1416 - 1424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mickelsen, C. Snyder, K. Trujillo, M. Bogue, D. B. Roth, and K. Meek Modulation of Terminal Deoxynucleotidyltransferase Activity by the DNA-Dependent Protein Kinase J. Immunol., July 15, 1999; 163(2): 834 - 843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C.M. Smith and S. P. Jackson The DNA-dependent protein kinase Genes & Dev., April 15, 1999; 13(8): 916 - 934. [Full Text]
|
|
|
|
 |
|
 J.M. SEKIGUCHI, Y. GAO, Y. GU, K. FRANK, Y. SUN, J. CHAUDHURI, C. ZHU, H.-L. CHENG, J. MANIS, D. FERGUSON, et al. Nonhomologous End-joining Proteins Are Required for V(D)J Recombination, Normal Growth, and Neurogenesis Cold Spring Harb Symp Quant Biol, January 1, 1999; 64(0): 169 - 182. [Abstract] [PDF]
|
|
|
|
|
|
M. A. Bogue, C. Jhappan, and D. B. Roth Analysis of variable (diversity) joining recombination in DNAdependent protein kinase (DNA-PK)-deficient mice reveals DNA-PK-independent pathways for both signal and coding joint formation PNAS, December 22, 1998; 95(26): 15559 - 15564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Munoz, M. Z. Zdzienicka, J.-M. Blanchard, and J. Piette Hypersensitivity of Ku-Deficient Cells toward the DNA Topoisomerase II Inhibitor ICRF-193 Suggests a Novel Role for Ku Antigen during the G2 and M Phases of the Cell Cycle Mol. Cell. Biol., October 1, 1998; 18(10): 5797 - 5808. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chen, K. V. Inamdar, P. Pfeiffer, E. Feldmann, M. F. Hannah, Y. Yu, J.-W. Lee, T. Zhou, S. P. Lees-Miller, and L. F. Povirk Accurate in Vitro End Joining of a DNA Double Strand Break with Partially Cohesive 3'-Overhangs and 3'-Phosphoglycolate Termini. EFFECT OF Ku ON REPAIR FIDELITY J. Biol. Chem., June 22, 2001; 276(26): 24323 - 24330. [Abstract] [Full Text] [PDF]
|
|