Nucleic Acids Research, Vol 26, Issue 15 3513-3520, Copyright © 1998 by Oxford University Press
E Peyretaillade, C Biderre, P Peyret, F Duffieux, G Metenier, M Gouy, B Michot and CP Vivares
Microsporidia are eukaryotic parasites lacking mitochondria, the ribosomes
of which present prokaryote-like features. In order to better understand
the structural evolution of rRNA molecules in microsporidia, the 5S and
rDNA genes were investigated in Encephalitozoon cuniculi . The genes are
not in close proximity. Non-tandemly arranged rDNA units are on every one
of the 11 chromosomes. Such a dispersion is also shown in two other
Encephalitozoon species. Sequencing of the 5S rRNA coding region reveals a
120 nt long RNA which folds according to the eukaryotic consensus
structural shape. In contrast, the LSU rRNA molecule is greatly reduced in
length (2487 nt). This dramatic shortening is essentially due to truncation
of divergent domains, most of them being removed. Most variable stems of
the conserved core are also deleted, reducing the LSU rRNA to only those
structural features preserved in all living cells. This suggests that the
E.cuniculi LSU rRNA performs only the basic mechanisms of translation. LSU
rRNA phylogenetic analysis with the BASEML program favours a relatively
recent origin of the fast evolving microsporidian lineage. Therefore, the
prokaryote-like ribosomal features, such as the absence of ITS2, may be
derived rather than primitive characters.
ARTICLES
Microsporidian Encephalitozoon cuniculi, a unicellular eukaryote with an unusual chromosomal dispersion of ribosomal genes and a LSU rRNA reduced to the universal core
Laboratoire de Protistologie Moleculaire et Cellulaire des Parasites Opportunistes, LBCP, UPESA CNRS 6023, Universite Blaise Pascal, 24 Avenue des Landais, 63177 Aubiere Cedex, France.
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