Nucleic Acids Research, Vol 26, Issue 17 4068-4077, Copyright © 1998 by Oxford University Press
X Qu, Z Yang, S Zhang, L Shen, AW Dangel, JH Hughes, KL Redman, LC Wu and CY Yu
The human helicase gene SKI2W is located between RD and RP1 in the class
III region of the major histocompatibility complex. Transcripts of SKI2W
are detectable in RNA samples isolated from multiple tissues. The protein
product Ski2w shares striking amino acid sequence similarities to the yeast
antiviral protein Ski2p that controls the translation of mRNAs, probably
based on the mRNA structural integrity. Whether this translational
regulation mechanism for cellular and viral RNAs exists in mammals is under
investigation. Antisera against human Ski2w were generated using fusion
proteins produced in bacteria or insect cells. Western blot analysis showed
that the endogenous Ski2w protein is approximately 140 kDa in size and is
enriched in polysomal fractions of cytoplasmic extracts from HeLa cells.
Ribosomal profile studies revealed that Ski2w distributed throughout the
entire sucrose gradient in the presence of Mg2+, but co-sedimented with the
18S rRNA- containing 40S subunit and the small ribosomal subunit protein
S27a in the presence of EDTA. The co-sedimentation of Ski2w with the 40S
subunit is not affected by RNase A treatment of the cell extract, or the
addition of KCl to 0.5 M, suggesting that Ski2w is associated with the 40S
ribosomal subunit. Indirect immunofluorescence experiments showed that
human Ski2w is localized in the nucleoli and in the cytoplasm. In essence,
human Ski2w is present at the sites of ribosome biogenesis and protein
synthesis.
ARTICLES
The human DEVH-box protein Ski2w from the HLA is localized in nucleoli and ribosomes
Children's Hospital Research Foundation, Molecular, Cellular and Developmental Biology Program, Department of Pediatrics, The Ohio StateUniversity, Columbus, OH 43205-2696, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. Groppo and A. C. Palmenberg Cardiovirus 2A Protein Associates with 40S but Not 80S Ribosome Subunits during Infection J. Virol., December 1, 2007; 81(23): 13067 - 13074. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hallier, J. Desreac, and B. Felden Small protein B interacts with the large and the small subunits of a stalled ribosome during trans-translation. Nucleic Acids Res., January 1, 2006; 34(6): 1935 - 1943. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Qing, Z. Qu, and G. Xiao Stabilization of Basally Translated NF-{kappa}B-inducing Kinase (NIK) Protein Functions as a Molecular Switch of Processing of NF-{kappa}B2 p100 J. Biol. Chem., December 9, 2005; 280(49): 40578 - 40582. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. I. ORBAN and E. IZAURRALDE Decay of mRNAs targeted by RISC requires XRN1, the Ski complex, and the exosome RNA, April 1, 2005; 11(4): 459 - 469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Searfoss, T. E. Dever, and R. Wickner Linking the 3' Poly(A) Tail to the Subunit Joining Step of Translation Initiation: Relations of Pab1p, Eukaryotic Translation Initiation Factor 5B (Fun12p), and Ski2p-Slh1p Mol. Cell. Biol., August 1, 2001; 21(15): 4900 - 4908. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Searfoss and R. B. Wickner 3' poly(A) is dispensable for translation PNAS, August 1, 2000; 97(16): 9133 - 9137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xue, X. Bai, I. Lee, G. Kallstrom, J. Ho, J. Brown, A. Stevens, and A. W. Johnson Saccharomyces cerevisiae RAI1 (YGL246c) Is Homologous to Human DOM3Z and Encodes a Protein That Binds the Nuclear Exoribonuclease Rat1p Mol. Cell. Biol., June 1, 2000; 20(11): 4006 - 4015. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. F. Zirwes, J. Eilbracht, S. Kneissel, and M. S. Schmidt-Zachmann A Novel Helicase-Type Protein in the Nucleolus: Protein NOH61 Mol. Biol. Cell, April 1, 2000; 11(4): 1153 - 1167. [Abstract] [Full Text]
|
|
|
|
|
|
Z. Yang, A. R. Mendoza, T. R. Welch, W. B. Zipf, and C. Y. Yu Modular Variations of the Human Major Histocompatibility Complex Class III Genes for Serine/Threonine Kinase RP, Complement Component C4, Steroid 21-Hydroxylase CYP21, and Tenascin TNX (the RCCX Module). A MECHANISM FOR GENE DELETIONS AND DISEASE ASSOCIATIONS J. Biol. Chem., April 23, 1999; 274(17): 12147 - 12156. [Abstract] [Full Text] [PDF]
|
|