Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (51K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Gupta, R.
Right arrow Articles by Rigby, P. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gupta, R.
Right arrow Articles by Rigby, P. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 26, Issue 19 4538-4539, Copyright © 1998 by Oxford University Press

ARTICLES

Isolation of developmentally regulated genes by differential display screening of cDNA libraries

R Gupta, P Thomas, RS Beddington and PW Rigby
Division of Eukaryotic Molecular Genetics and Division of Mammalian Development,MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.

mRNA differential display RT-PCR has been extensively used for the isolation of genes differentially expressed between RNA populations. We have assessed its utility for the identification of developmentally regulated genes in plasmid cDNA libraries derived from individual tissues dissected from early mouse embryos. Using plasmid Southern blot hybridisation as a secondary screen, we are able to identify such genes and show by whole-mount in situ hybridisation that their expression pattern is that expected from the differential display profile.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Clin. Microbiol. Rev.Home page
J. Sturtevant
Applications of Differential-Display Reverse Transcription-PCR to Molecular Pathogenesis and Medical Mycology
Clin. Microbiol. Rev., July 1, 2000; 13(3): 408 - 427.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
A. K. Singh and L. A. Sherman
Identification of Iron-Responsive, Differential Gene Expression in the Cyanobacterium Synechocystis sp. Strain PCC 6803 with a Customized Amplification Library
J. Bacteriol., June 15, 2000; 182(12): 3536 - 3543.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.