Nucleic Acids Research, Vol 26, Issue 2 638-644, Copyright © 1998 by Oxford University Press
S Nakajima, M Sugiyama, S Iwai, K Hitomi, E Otoshi, ST Kim, CZ Jiang, T Todo, AB Britt and K Yamamoto
UV radiation induces two major classes of pyrimidine dimers: the pyrimidine
[6-4] pyrimidone photoproduct (6-4 product) and the cyclobutane pyrimidine
dimer (CPD). Many organisms produce enzymes, termed photolyases, that
specifically bind to these damage products and split them via a UV-A/blue
light-dependent mechanism, thereby reversing the damage. These photolyases
are specific for either CPDs or 6-4 products. A gene that expresses a
protein with 6-4 photolyase activity in vitro was recently cloned from
Drosophila melanogaster and Xenopus laevis. We report here the isolation of
a homolog of this gene, cloned on the basis of sequence similarity, from
the higher plant Arabidopsis thaliana. This cloned gene produces a protein
with 6-4 photolyase activity when expressed in Escherichia coli. We also
find that a previously described mutant of Arabidopsis (uvr3) that is
defective in photoreactivation of 6-4 products carries a nonsense mutation
in this 6- 4 photolyase homolog. We have therefore termed this gene UVR3.
Although homologs of this gene have previously been shown to produce a
functional 6-4 photolyase when expressed in heterologous systems, this is
the first demonstration of a requirement for this gene for
photoreactivation of 6-4 products in vivo.
ARTICLES
Cloning and characterization of a gene (UVR3) required for photorepair of 6-4 photoproducts in Arabidopsis thaliana
Biological Institute, Graduate School of Science, Tohoku University, Sendai 980-77, Japan.
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