Nucleic Acids Research, Vol 26, Issue 2 655-661, Copyright © 1998 by Oxford University Press
A Verschoor, JR Warner, S Srivastava, RA Grassucci and J Frank
The 80S ribosome from Saccharomyces cerevisiae has been reconstructed from
cryo electron micrographs to a resolution of 35 A. It is strikingly similar
to the 70S ribosome from Escherichia coli, while displaying the
characteristic eukaryotic features familiar from reconstructions of
ribosomes from higher eukaryotes. Aside from the elaboration of a number of
peripherally located features on the two subunits and greater overall size,
the largest difference between the yeast and E.coli ribosomes is in a mass
increase on one side of the large (60S) subunit. It thus appears more
elliptical than the characteristically globular 50S subunit from E.coli.
The interior of the 60S subunit reveals a variable diameter tunnel spanning
the subunit between the interface canyon and a site on the lower back of
the subunit, presumably the exit site through which the nascent polypeptide
chain emerges from the ribosome.
ARTICLES
Three-dimensional structure of the yeast ribosome
Wadsworth Center, New York State Department of Health, Empire State Plaza, PO Box 509, Albany, NY 12201-0509, USA. adri@orkney.ph.albany.edu
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Wang, C. Li, E. Wang, and X. Wang Uncovering the rules for protein-protein interactions from yeast genomic data PNAS, March 10, 2009; 106(10): 3752 - 3757. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Champion, B. H. Lane, M. E. Jackrel, L. Regan, and S. J. Baserga A Direct Interaction between the Utp6 Half-a-Tetratricopeptide Repeat Domain and a Specific Peptide in Utp21 Is Essential for Efficient Pre-rRNA Processing Mol. Cell. Biol., November 1, 2008; 28(21): 6547 - 6556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. I. Moy and P. A. Silver Requirements for the nuclear export of the small ribosomal subunit J. Cell Sci., July 15, 2002; 115(14): 2985 - 2995. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-W. Lee, S. J. Berger, S. Martinovic', L. Pasa-Tolic', G. A. Anderson, Y. Shen, R. Zhao, and R. D. Smith Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR PNAS, April 30, 2002; 99(9): 5942 - 5947. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. BECKMANN, C.M.T. SPAHN, J. FRANK, and G. BLOBEL The Active 80S Ribosome-Sec61 Complex Cold Spring Harb Symp Quant Biol, January 1, 2001; 66(0): 543 - 554. [Abstract] [PDF]
|
|
|
|
|
|
A.W. JOHNSON, J.H.-N. HO, G. KALLSTROM, C. TROTTA, E. LUND, L. KAHAN, J. DAHLBERG, and J. HEDGES Nuclear Export of the Large Ribosomal Subunit Cold Spring Harb Symp Quant Biol, January 1, 2001; 66(0): 599 - 606. [Abstract] [PDF]
|
|
|
|
 |
|
 D. Nadano and T.-A. Sato Caspase-3-dependent and -independent Degradation of 28 S Ribosomal RNA May Be Involved in the Inhibition of Protein Synthesis during Apoptosis Initiated by Death Receptor Engagement J. Biol. Chem., April 28, 2000; 275(18): 13967 - 13973. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. I. Moy and P. A. Silver Nuclear export of the small ribosomal subunit requires the Ran-GTPase cycle and certain nucleoporins Genes & Dev., August 15, 1999; 13(16): 2118 - 2133. [Abstract] [Full Text]
|
|
|
|
|
|
M. C. Justice, T. Ku, M.-J. Hsu, K. Carniol, D. Schmatz, and J. Nielsen Mutations in Ribosomal Protein L10e Confer Resistance to the Fungal-specific Eukaryotic Elongation Factor 2 Inhibitor Sordarin J. Biol. Chem., February 19, 1999; 274(8): 4869 - 4875. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. V. Rodnina and W. Wintermeyer Form follows function: Structure of an elongation factor G-ribosome complex PNAS, June 23, 1998; 95(13): 7237 - 7239. [Full Text] [PDF]
|
|