Nucleic Acids Research, Vol 26, Issue 20 4771-4777, Copyright © 1998 by Oxford University Press
CC Yang, OI Ornatsky, JC McDermott, TF Cruz and CA Prody
Myocyte enhancer factor 2 (MEF2) has been implicated in the complex
hierarchical regulation of muscle-specific gene expression and
differentiation. While the MyoD family members are able to initiate the
skeletal muscle differentiation program, whether MEF2 is sufficient in
directing skeletal muscle differentiation is still controversial.
Furthermore, how MEF2 transactivates its target genes is not fully
understood. It has been suggested that the interactions of MEF2 with other
factors modify its transcriptional activity. Therefore, the identification
of MEF2-interacting factors may be important in understanding the mechanism
by which MEF2 activates its target genes. In this study, a
mitogen-activated protein kinase (MAP kinase), ERK5/BMK1 was found to
interact with MEF2 in a yeast two hybrid screen. The interaction was
confirmed by a glutathione S -transferase-pull down assay and a
co-immunoprecipitation study indicating that endogenous ERK5 and MEF2
interact with each other in vivo . The interacting domain of MEF2 was
mapped to the N-terminus which contains the highly conserved MADS and MEF2
domains. Functionally, ERK5/BMK1 was able to phosphorylate MEF2 in vitro .
Furthermore, when cotransfected with ERK5/BMK1, the transactivation
capacity of MEF2 was enhanced. These results suggest that the functions of
MEF2 could be regulated through ERK5/BMK1.
ARTICLES
Interaction of myocyte enhancer factor 2 (MEF2) with a mitogen- activated protein kinase, ERK5/BMK1
Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. L. S. Perry, C. Yang, N. Soora, J. Salma, M. Marback, L. Naghibi, H. Ilyas, J. Chan, J. W. Gordon, and J. C. McDermott Direct Interaction between Myocyte Enhancer Factor 2 (MEF2) and Protein Phosphatase 1{alpha} Represses MEF2-Dependent Gene Expression Mol. Cell. Biol., June 15, 2009; 29(12): 3355 - 3366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Du, R. L. S. Perry, N. B. Nowacki, J. W. Gordon, J. Salma, J. Zhao, A. Aziz, J. Chan, K. W. M. Siu, and J. C. McDermott Protein Kinase A Represses Skeletal Myogenesis by Targeting Myocyte Enhancer Factor 2D Mol. Cell. Biol., May 1, 2008; 28(9): 2952 - 2970. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Morimoto, K. Kondoh, S. Nishimoto, K. Terasawa, and E. Nishida Activation of a C-terminal Transcriptional Activation Domain of ERK5 by Autophosphorylation J. Biol. Chem., December 7, 2007; 282(49): 35449 - 35456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C. W. Groenendijk, K. Van der Heiden, B. P. Hierck, and R. E. Poelmann The Role of Shear Stress on ET-1, KLF2, and NOS-3 Expression in the Developing Cardiovascular System of Chicken Embryos in a Venous Ligation Model Physiology, December 1, 2007; 22(6): 380 - 389. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Potthoff and E. N. Olson MEF2: a central regulator of diverse developmental programs Development, December 1, 2007; 134(23): 4131 - 4140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. B. Atkins and M. K. Jain Role of Kruppel-Like Transcription Factors in Endothelial Biology Circ. Res., June 22, 2007; 100(12): 1686 - 1695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gregoire, L. Xiao, J. Nie, X. Zhang, M. Xu, J. Li, J. Wong, E. Seto, and X.-J. Yang Histone Deacetylase 3 Interacts with and Deacetylates Myocyte Enhancer Factor 2 Mol. Cell. Biol., February 15, 2007; 27(4): 1280 - 1295. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, B. Su, and Z. Xia Brain-derived Neurotrophic Factor Activates ERK5 in Cortical Neurons via a Rap1-MEKK2 Signaling Cascade J. Biol. Chem., November 24, 2006; 281(47): 35965 - 35974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. W. Truman, S. H. Millson, J. M. Nuttall, V. King, M. Mollapour, C. Prodromou, L. H. Pearl, and P. W. Piper Expressed in the Yeast Saccharomyces cerevisiae, Human ERK5 Is a Client of the Hsp90 Chaperone That Complements Loss of the Slt2p (Mpk1p) Cell Integrity Stress-Activated Protein Kinase Eukaryot. Cell, November 1, 2006; 5(11): 1914 - 1924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-h. Feng and S. C. Kenney Valproic Acid Enhances the Efficacy of Chemotherapy in EBV-Positive Tumors by Increasing Lytic Viral Gene Expression. Cancer Res., September 1, 2006; 66(17): 8762 - 8769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Schweppe, T. H. Cheung, and N. G. Ahn Global Gene Expression Analysis of ERK5 and ERK1/2 Signaling Reveals a Role for HIF-1 in ERK5-mediated Responses J. Biol. Chem., July 28, 2006; 281(30): 20993 - 21003. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kondoh, K. Terasawa, H. Morimoto, and E. Nishida Regulation of nuclear translocation of extracellular signal-regulated kinase 5 by active nuclear import and export mechanisms. Mol. Cell. Biol., March 1, 2006; 26(5): 1679 - 1690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gregoire, A. M. Tremblay, L. Xiao, Q. Yang, K. Ma, J. Nie, Z. Mao, Z. Wu, V. Giguere, and X.-J. Yang Control of MEF2 Transcriptional Activity by Coordinated Phosphorylation and Sumoylation J. Biol. Chem., February 17, 2006; 281(7): 4423 - 4433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Sohn, D. Li, L. K. Lee, and A. Winoto Transcriptional Regulation of Tissue-Specific Genes by the ERK5 Mitogen-Activated Protein Kinase Mol. Cell. Biol., October 1, 2005; 25(19): 8553 - 8566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Nakagawa, M. Arnold, M. Nakagawa, H. Hamada, J. M. Shelton, H. Kusano, T. M. Harris, G. Childs, K. P. Campbell, J. A. Richardson, et al. Centronuclear myopathy in mice lacking a novel muscle-specific protein kinase transcriptionally regulated by MEF2 Genes & Dev., September 1, 2005; 19(17): 2066 - 2077. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. W. Baker, K. K. K. Tanaka, N. Klitgord, and R. M. Cripps Adult Myogenesis in Drosophila melanogaster Can Proceed Independently of Myocyte Enhancer Factor-2 Genetics, August 1, 2005; 170(4): 1747 - 1759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-M. Sturla, C. W. Cowan, L. Guenther, R. C. Castellino, J. Y.H. Kim, and S. L. Pomeroy A Novel Role for Extracellular Signal-Regulated Kinase 5 and Myocyte Enhancer Factor 2 in Medulloblastoma Cell Death Cancer Res., July 1, 2005; 65(13): 5683 - 5689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Carvajal-Vergara, S. Tabera, J. C. Montero, A. Esparis-Ogando, R. Lopez-Perez, G. Mateo, N. Gutierrez, M. Parmo-Cabanas, J. Teixido, J. F. San Miguel, et al. Multifunctional role of Erk5 in multiple myeloma Blood, June 1, 2005; 105(11): 4492 - 4499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Grey, A. Mery, and M. Puceat Fine-tuning in Ca2+ homeostasis underlies progression of cardiomyopathy in myocytes derived from genetically modified embryonic stem cells Hum. Mol. Genet., May 15, 2005; 14(10): 1367 - 1377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Millson, A. W. Truman, V. King, C. Prodromou, L. H. Pearl, and P. W. Piper A Two-Hybrid Screen of the Yeast Proteome for Hsp90 Interactors Uncovers a Novel Hsp90 Chaperone Requirement in the Activity of a Stress-Activated Mitogen-Activated Protein Kinase, Slt2p (Mpk1p) Eukaryot. Cell, May 1, 2005; 4(5): 849 - 860. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Barros and C. J. Marshall Activation of either ERK1/2 or ERK5 MAP kinase pathways can lead to disruption of the actin cytoskeleton J. Cell Sci., April 15, 2005; 118(8): 1663 - 1671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gregoire and X.-J. Yang Association with Class IIa Histone Deacetylases Upregulates the Sumoylation of MEF2 Transcription Factors Mol. Cell. Biol., March 15, 2005; 25(6): 2273 - 2287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Edmunds and L. C. Mahadevan MAP kinases as structural adaptors and enzymatic activators in transcription complexes J. Cell Sci., September 1, 2004; 117(17): 3715 - 3723. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kuida and D. M. Boucher Functions of MAP Kinases: Insights from Gene-Targeting Studies J. Biochem., June 1, 2004; 135(6): 653 - 656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Raviv, E. Kalie, and R. Seger MEK5 and ERK5 are localized in the nuclei of resting as well as stimulated cells, while MEKK2 translocates from the cytosol to the nucleus upon stimulation J. Cell Sci., May 1, 2004; 117(9): 1773 - 1784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zheng, G. Yin, C. Yan, M. Cavet, and B. C. Berk 14-3-3{beta} Binds to Big Mitogen-activated Protein Kinase 1 (BMK1/ERK5) and Regulates BMK1 Function J. Biol. Chem., March 5, 2004; 279(10): 8787 - 8791. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Liu, J. E. Cavanaugh, Y. Wang, H. Sakagami, Z. Mao, and Z. Xia ERK5 activation of MEF2-mediated gene expression plays a critical role in BDNF-promoted survival of developing but not mature cortical neurons PNAS, July 8, 2003; 100(14): 8532 - 8537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Akazawa and I. Komuro Roles of Cardiac Transcription Factors in Cardiac Hypertrophy Circ. Res., May 30, 2003; 92(10): 1079 - 1088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Cox, M. Du, M. Marback, E. C. C. Yang, J. Chan, K. W. M. Siu, and J. C. McDermott Phosphorylation Motifs Regulating the Stability and Function of Myocyte Enhancer Factor 2A J. Biol. Chem., April 18, 2003; 278(17): 15297 - 15303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Sohn, B. K. Sarvis, D. Cado, and A. Winoto ERK5 MAPK Regulates Embryonic Angiogenesis and Acts as a Hypoxia-sensitive Repressor of Vascular Endothelial Growth Factor Expression J. Biol. Chem., November 1, 2002; 277(45): 43344 - 43351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Buschbeck, J. Eickhoff, M. N. Sommer, and A. Ullrich Phosphotyrosine-specific Phosphatase PTP-SL Regulates the ERK5 Signaling Pathway J. Biol. Chem., August 9, 2002; 277(33): 29503 - 29509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. P. Regan, W. Li, D. M. Boucher, S. Spatz, M. S. Su, and K. Kuida Erk5 null mice display multiple extraembryonic vascular and embryonic cardiovascular defects PNAS, July 9, 2002; 99(14): 9248 - 9253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Li, M. Puceat, C. Perez-Terzic, A. Mery, K. Nakamura, M. Michalak, K.-H. Krause, and M. E. Jaconi Calreticulin reveals a critical Ca2+ checkpoint in cardiac myofibrillogenesis J. Cell Biol., July 8, 2002; 158(1): 103 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Esparis-Ogando, E. Diaz-Rodriguez, J. C. Montero, L. Yuste, P. Crespo, and A. Pandiella Erk5 Participates in Neuregulin Signal Transduction and Is Constitutively Active in Breast Cancer Cells Overexpressing ErbB2 Mol. Cell. Biol., January 1, 2002; 22(1): 270 - 285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Li, D. A. Linseman, M. P. Allen, M. K. Meintzer, X. Wang, T. Laessig, M. E. Wierman, and K. A. Heidenreich Myocyte Enhancer Factor 2A and 2D Undergo Phosphorylation and Caspase-Mediated Degradation during Apoptosis of Rat Cerebellar Granule Neurons J. Neurosci., September 1, 2001; 21(17): 6544 - 6552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Miska, E. Langley, D. Wolf, C. Karlsson, J. Pines, and T. Kouzarides Differential localization of HDAC4 orchestrates muscle differentiation Nucleic Acids Res., August 15, 2001; 29(16): 3439 - 3447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. A. Quinn, C.-C. Yang, J. L. Wrana, and J. C. McDermott Smad proteins function as co-modulators for MEF2 transcriptional regulatory proteins Nucleic Acids Res., February 1, 2001; 29(3): 732 - 742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Cavanaugh, J. Ham, M. Hetman, S. Poser, C. Yan, and Z. Xia Differential Regulation of Mitogen-Activated Protein Kinases ERK1/2 and ERK5 by Neurotrophins, Neuronal Activity, and cAMP in Neurons J. Neurosci., January 15, 2001; 21(2): 434 - 443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. G. Kasler, J. Victoria, O. Duramad, and A. Winoto ERK5 Is a Novel Type of Mitogen-Activated Protein Kinase Containing a Transcriptional Activation Domain Mol. Cell. Biol., November 15, 2000; 20(22): 8382 - 8389. [Abstract] [Full Text]
|
|
|
|
|
|
A. G. Ridgeway, S. Wilton, and I. S. Skerjanc Myocyte Enhancer Factor 2C and Myogenin Up-regulate Each Other's Expression and Induce the Development of Skeletal Muscle in P19 Cells J. Biol. Chem., January 7, 2000; 275(1): 41 - 46. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Yang, A. Galanis, and A. D. Sharrocks Targeting of p38 Mitogen-Activated Protein Kinases to MEF2 Transcription Factors Mol. Cell. Biol., June 1, 1999; 19(6): 4028 - 4038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Marinissen, M. Chiariello, M. Pallante, and J. S. Gutkind A Network of Mitogen-Activated Protein Kinases Links G Protein-Coupled Receptors to the c-jun Promoter: a Role for c-Jun NH2-Terminal Kinase, p38s, and Extracellular Signal-Regulated Kinase 5 Mol. Cell. Biol., June 1, 1999; 19(6): 4289 - 4301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kato, M. Zhao, A. Morikawa, T. Sugiyama, D. Chakravortty, N. Koide, T. Yoshida, R. I. Tapping, Y. Yang, T. Yokochi, et al. Big Mitogen-activated Kinase Regulates Multiple Members of the MEF2 Protein Family J. Biol. Chem., June 9, 2000; 275(24): 18534 - 18540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Sun, K. Kesavan, B. C. Schaefer, T. P. Garrington, M. Ware, N. L. Johnson, E. W. Gelfand, and G. L. Johnson MEKK2 Associates with the Adapter Protein Lad/RIBP and Regulates the MEK5-BMK1/ERK5 Pathway J. Biol. Chem., February 9, 2001; 276(7): 5093 - 5100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Tamir and E. Bengal Phosphoinositide 3-Kinase Induces the Transcriptional Activity of MEF2 Proteins during Muscle Differentiation J. Biol. Chem., October 27, 2000; 275(44): 34424 - 34432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Dong, J. S. Gutkind, and A. C. Larner Granulocyte Colony-stimulating Factor Induces Erk5 Activation, Which Is Differentially Regulated by Protein-tyrosine Kinases and Protein Kinase C. REGULATION OF CELL PROLIFERATION AND SURVIVAL J. Biol. Chem., March 30, 2001; 276(14): 10811 - 10816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. V. Kumar and L. R. Bernstein Ten ERK-related Proteins in Three Distinct Classes Associate with AP-1 Proteins and/or AP-1 DNA J. Biol. Chem., August 17, 2001; 276(34): 32362 - 32372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Dunn, A. R. Simard, R. Bassel-Duby, R. S. Williams, and R. N. Michel Nerve Activity-dependent Modulation of Calcineurin Signaling in Adult Fast and Slow Skeletal Muscle Fibers J. Biol. Chem., November 21, 2001; 276(48): 45243 - 45254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lu, T. A. McKinsey, R. L. Nicol, and E. N. Olson Signal-dependent activation of the MEF2 transcription factor by dissociation from histone deacetylases PNAS, April 11, 2000; 97(8): 4070 - 4075. [Abstract] [Full Text] [PDF]
|
|