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Nucleic Acids Research, Vol 26, Issue 21 4917-4918, Copyright © 1998 by Oxford University Press

ARTICLES

AbeI, a restriction endonuclease from Azotobacter beijerinckii, which recognizes the asymmetric heptanucleotide sequence 5'-CCTCAGC-3'(-5/-2)

J Vitkute, Z Maneliene and A Janulaitis
Institute of Biotechnology, Graiciuno 8, 2028 Vilnius, Lithuania.

A new restriction endonuclease Abe I has beenisolated from Azotobacter beijerinckii. This enzymerecognizes the asymmetric heptanucleotide sequence 5'-CCTCAGC-3' and cleaves within it symmetrically at positions -5/-2 in the opposing strands, producing three base protruding 5'-ends.
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