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Nucleic Acids Research, Vol 26, Issue 22 5225-5227, Copyright © 1998 by Oxford University Press

ARTICLES

The use of tailed octamer primers for cycle sequencing

S Ball, MA Reeve, PS Robinson, F Hill, DM Brown and D Loakes
Nycomed Amersham plc, Amersham Laboratories, White Lion Road, Amersham, Buckinghamshire HP7 9LL, UK.

Studies have been carried out on the use of octamer oligonucleotides tailed with different base analogues as primers in cycle sequencing reactions. 5-Nitroindole tails improved the performance as primers of a number of octamers. A tail length of three or four 5-nitroindole residues significantly increased the sequencing signal intensity for almost all primers. The use of incomplete libraries of tailed octamer primers for primer walking is discussed.
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