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Nucleic Acids Research, Vol 26, Issue 22 5233-5234, Copyright © 1998 by Oxford University Press

ARTICLES

Detection and quantification of apoptosis in transiently transfected adherent cells

P Lassus and U Hibner
Institut de Genetique Moleculaire, CNRS UMR 5535, 1919 route de Mende, 34293 Montpellier Cedex 5, France. lassus@jones.cnrs-mop.fr

Apoptosis is a highly conserved form of cell death present in all eukaryotic cell types and controlled by multiple genes. Several methods have been developed to quantify apoptosis, but none is adapted for all cell types. It is particularly difficult to reliably assay apoptosis of adherent cells. We describe a new, rapid and reliable flow cytometric method which can be used for quantifiying apoptosis in a sub-population of transiently transfected adherent cells. This technique is based on the detection of transfected cells and the apoptotic sub-population by immunofluorescence and Annexin-V labelling, respectively.
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