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Nucleic Acids Research, Vol 26, Issue 4 1124-1125, Copyright © 1998 by Oxford University Press

ARTICLES

Functional complementation by electroporation of human BACs into mammalian fibroblast cells

JA Hejna, PL Johnstone, SL Kohler, DA Bruun, CA Reifsteck, SB Olson and RE Moses
Department of Molecular and Medical Genetics, L103, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, OR 97201, USA.

Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in situ hybridization, PCR and Southern hybridization.
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