Nucleic Acids Research, Vol 26, Issue 7 1775-1783, Copyright © 1998 by Oxford University Press
A Levitan, Yx Xu, C Ben-Dov, H Ben-Shlomo, Y Zhang and S Michaeli
Trypanosomes possess unique RNA processing mechanisms including trans-
splicing of pre-mRNA and RNA editing of mitochondrial transcripts. The
previous finding of a trimethylguanosine (TMG) capped U3 homologue in
trypanosomes suggests that rRNA processing may be related to the processing
in other eukaryotes. In this study, we describe the first trypanosomatid
snoRNA that belongs to the snoRNAs that were shown to guide ribose
methylation of rRNA. The RNA, identified in the monogenetic trypanosomatid
Leptomonas collosoma, was termed snoRNA-2 and is encoded by a multi-copy
gene. SnoRNA-2 is 85 nt long, it lacks a 5' cap and possesses the C and D
boxes characteristic to all snoRNAs that bind fibrillarin. Computer
analysis indicates a potential for base- pairing between snoRNA-2 and 5.8S
rRNA, and 18S rRNA. The putative interaction domains obey the rules
suggested for the interaction of guide snoRNA with its rRNA target for
directing ribose methylation on the rRNA. However, mapping the methylated
sites on the 5.8S rRNA and 18S rRNA indicates that the expected site on the
5.8S is methylated, whereas the site on the 18S is not. The proposed
interaction with 5.8S rRNA is further supported by the presence of psoralen
cross-link sites on snoRNA-2. GenBank search suggests that snoRNA-2 is not
related to any published snoRNAs. Because of the early divergence of the
Trypanosomatidae from the eukaryotic lineage, the presence of a methylating
snoRNA that is encoded by a multi-copy gene suggests that methylating
snoRNAs may have evolved in evolution from self-transcribed genes.
ARTICLES
Characterization of a novel trypanosomatid small nucleolar RNA
Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Lustig, C. Wachtel, M. Safro, L. Liu, and S. Michaeli 'RNA walk' a novel approach to study RNA-RNA interactions between a small RNA and its target Nucleic Acids Res., October 23, 2009; (2009) gkp872v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Barth, B. Shalem, A. Hury, I. D. Tkacz, X.-h. Liang, S. Uliel, I. Myslyuk, T. Doniger, M. Salmon-Divon, R. Unger, et al. Elucidating the Role of C/D snoRNA in rRNA Processing and Modification in Trypanosoma brucei Eukaryot. Cell, January 1, 2008; 7(1): 86 - 101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-H. LIANG, S. ULIEL, A. HURY, S. BARTH, T. DONIGER, R. UNGER, and S. MICHAELI A genome-wide analysis of C/D and H/ACA-like small nucleolar RNAs in Trypanosoma brucei reveals a trypanosome-specific pattern of rRNA modification RNA, May 1, 2005; 11(5): 619 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-h. Liang, A. Ochaion, Y.-x. Xu, Q. Liu, and S. Michaeli Small Nucleolar RNA Clusters in Trypanosomatid Leptomonas collosoma: GENOME ORGANIZATION, EXPRESSION STUDIES, AND THE POTENTIAL ROLE OF SEQUENCES PRESENT UPSTREAM FROM THE FIRST REPEATED CLUSTER J. Biol. Chem., February 13, 2004; 279(7): 5100 - 5109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-h. Liang, Q. Liu, and S. Michaeli Small nucleolar RNA interference induced by antisense or double-stranded RNA in trypanosomatids PNAS, June 24, 2003; 100(13): 7521 - 7526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liu, H. Ben-Shlomo, Y.-x. Xu, M. Z. Stern, I. Goncharov, Y. Zhang, and S. Michaeli The Trypanosomatid Signal Recognition Particle Consists of Two RNA Molecules, a 7SL RNA Homologue and a Novel tRNA-like Molecule J. Biol. Chem., May 9, 2003; 278(20): 18271 - 18280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-h. Liang, L. Liu, and S. Michaeli Identification of the First Trypanosome H/ACA RNA That Guides Pseudouridine Formation on rRNA J. Biol. Chem., October 19, 2001; 276(43): 40313 - 40318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Dunbar, A. A. Chen, S. Wormsley, and S. J. Baserga The genes for small nucleolar RNAs in Trypanosoma brucei are organized in clusters and are transcribed as a polycistronic RNA Nucleic Acids Res., August 1, 2000; 28(15): 2855 - 2861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Li, L. R. Otake, Y.-x. Xu, and S. Michaeli The trans-Spliceosomal U4 RNA from the Monogenetic Trypanosomatid Leptomonas collosoma. CLONING AND IDENTIFICATION OF A TRANSCRIBED tRNA-LIKE ELEMENT THAT CONTROLS ITS EXPRESSION J. Biol. Chem., January 28, 2000; 275(4): 2259 - 2264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ben-Shlomo, A. Levitan, N. E. Shay, I. Goncharov, and S. Michaeli RNA Editing Associated with the Generation of Two Distinct Conformations of the Trypanosomatid Leptomonas collosoma 7SL RNA J. Biol. Chem., September 3, 1999; 274(36): 25642 - 25650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Dunbar, S. Wormsley, T. M. Lowe, and S. J. Baserga Fibrillarin-associated Box C/D Small Nucleolar RNAs in Trypanosoma brucei. SEQUENCE CONSERVATION AND IMPLICATIONS FOR 2'-O-RIBOSE METHYLATION OF rRNA J. Biol. Chem., May 5, 2000; 275(19): 14767 - 14776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-x. Xu, L. Liu, C. Lopez-Estrano, and S. Michaeli Expression Studies on Clustered Trypanosomatid Box C/D Small Nucleolar RNAs J. Biol. Chem., April 20, 2001; 276(17): 14289 - 14298. [Abstract] [Full Text] [PDF]
|
|