Nucleic Acids Research, Vol 26, Issue 7 1848-1850, Copyright © 1998 by Oxford University Press
RD Kirsch and E Joly
The QuikChangeTM protocol is one of the simplest and fastest methods for
site-directed mutagenesis, but introduces mutations at only one site at a
time, and requires two HPLC-purified complementary oligonucleotides. Here,
we describe that this method can be used with non-overlapping
oligonucleotides. By doing this, two separate sites can be mutagenised
simultaneously, or money can be saved by using a second 'standard'
oligonucleotide. By a further modification, we have also used the
QuikChangeTM approach to exchange DNA sequences between closely related
genes.
ARTICLES
An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes
The Babraham Institute, Babraham, Cambridge CB2 4AT, UK.
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