Nucleic Acids Research, Vol 27, Issue 15 3173-3182, Copyright © 1999 by Oxford University Press
RG Keene, A Mueller, R Landick and L London
Using either highly purified RNA polymerase II (pol II) elongation
complexes assembled on oligo(dC)-tailed templates or promoter-initiated
(extract-generated) pol II elongation complexes, the precise 3" ends of
transcripts produced during transcription in vitro at several human c- and
N- myc pause, arrest and termination sites were determined. Despite a low
overall similarity between the entire c- and N- myc first exon sequences,
many positions of pol II pausing, arrest or termination occurred within
short regions of related sequence shared between the c- and N- myc
templates. The c- and N- myc genes showed three general classes of sequence
conservation near intrinsic pause, arrest or termination sites: (i) sites
where arrest or termination occurred after the synthesis of runs of
uridines (Us) preceding the transcript 3" end, (ii) sites downstream of
potential RNA hairpins and (iii) sites after nucleotide addition following
either a U or a C or following a combination of several pyrimidines near
the transcript 3" end. The finding that regions of similarity occur near
the sites of pol II pausing, arrest or termination suggests that the
mechanism of c- and N- myc regulation at the level of transcript elongation
may be similar and not divergent as previously proposed.
ARTICLES
Transcriptional pause, arrest and termination sites for RNA polymerase II in mammalian N- and c-myc genes
Department of Biology, Washington University, St. Louis, MO 63130, USA.
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