Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (492K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Keller, R. C.
Right arrow Articles by Sogo, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Keller, R. C.
Right arrow Articles by Sogo, J. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 27, Issue 17 3433-3437, Copyright © 1999 by Oxford University Press

ARTICLES

Electron microscopic analysis reveals that replication factor C is sequestered by single-stranded DNA

RC Keller, R Mossi, G Maga, RE Wellinger, U Hubscher and JM Sogo
Institute for Cell Biology, ETH-Honggerberg, CH-8049 Zurich, Switzerland.

Replication factor C (RF-C) is a eukaryotic heteropentameric protein required for DNA replication and repair processes by loading proliferating cell nuclear antigen (PCNA) onto DNA in an ATP-dependent manner. Prior to loading PCNA, RF-C binds to DNA. This binding is thought to be restricted to a specific DNA structure, namely to a primer/template junction. Using the electron microscope we have examined the affinity of human heteropentameric RF-C and the DNA- binding region within the large subunit of RF-C from Drosophila melanogaster (dRF-Cp140) to heteroduplex DNA. The electron microscopic data indicate that both human heteropentameric RF-C and the DNA-binding region within dRF-Cp140 are sequestered by single-stranded DNA. No preferential affinity for the 3' or 5' transition points from single- to double-stranded DNA was evident.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
R. Pelletier, M. M. Krasilnikova, G. M. Samadashwily, R. Lahue, and S. M. Mirkin
Replication and Expansion of Trinucleotide Repeats in Yeast
Mol. Cell. Biol., February 15, 2003; 23(4): 1349 - 1357.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. M. Hingorani and M. M. Coman
On the Specificity of Interaction between the Saccharomyces cerevisiae Clamp Loader Replication Factor C and Primed DNA Templates during DNA Replication
J. Biol. Chem., November 27, 2002; 277(49): 47213 - 47224.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
I. K. O. Cann, S. Ishino, M. Yuasa, H. Daiyasu, H. Toh, and Y. Ishino
Biochemical Analysis of Replication Factor C from the Hyperthermophilic Archaeon Pyrococcus furiosus
J. Bacteriol., April 15, 2001; 183(8): 2614 - 2623.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
M. Stucki, Z. O. Jonsson, and U. Hubscher
In Eukaryotic Flap Endonuclease 1, the C Terminus Is Essential for Substrate Binding
J. Biol. Chem., March 9, 2001; 276(11): 7843 - 7849.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.