Nucleic Acids Research, Vol 27, Issue 17 e16-e16, Copyright © 1999 by Oxford University Press
PO Angrand, N Daigle, F van der Hoeven, HR Scholer and AF Stewart
ET recombination is a way to engineer DNA in Escherichia coli using
homologous recombination. Here we develop the potential of ET recombination
in two ways relevant to complex engineering exercises such as building gene
targeting constructs. First, a targeting construct was made in a single
step. Second, ET recombination was used to place two unique restriction
sites at precise positions in a large genomic clone. Subsequently a complex
targeting construct was created by ligation with a multifunctional
cassette.
ARTICLES
Simplified generation of targeting constructs using ET recombination
Gene Expression Program and Germ Cell Biology Laboratory, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69177 Heidelberg, Germany.
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