Nucleic Acids Research, Vol 27, Issue 19 3779-3791, Copyright © 1999 by Oxford University Press
P Rollini, SJ Namciu, MD Marsden and RE Fournier
Matrix-attachment regions (MARs) are DNA elements that are defined by their
abilities to bind to isolated nuclear matrices in vitro. The DNA sequences
of different matrix-binding elements vary widely. The locations of some
MARs at the ends of chromatin loops suggest that they may represent
boundaries of individual chromatin domains. As such, MARs may play
important roles in regulating transcription and chromatin structure. As a
first step towards assessing the roles of MARs in these processes, we
assayed DNA sequences from the human serine protease inhibitor (serpin)
gene cluster at 14q32.1 for matrix-binding activity in vitro. This
approximately 150 kb region contains the cell-specific genes encoding
alpha1-anti-trypsin (alpha1AT) and corticosteroid- binding globulin (CBG),
as well as an antitrypsin-related sequence termed ATR. A DNase
I-hypersensitive site (DHS) map of the locus has recently been described.
We report here that the alpha1AT-ATR-CBG region contains five distinct
MARs. There is a strong matrix-binding element approximately 16 kb upstream
of alpha1AT; three MARs are between ATR and CBG and one MAR is within the
CBG gene itself. These MARs were matrix-associated in all cell types
examined. DNA sequencing indicated that the serpin MARs contained
predominantly repetitive DNA, although the types of DNA repeats differed
among the MARs.
ARTICLES
Identification and characterization of nuclear matrix-attachment regions in the human serpin gene cluster at 14q32.1
Division of Basic Sciences, A2-025, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, PO Box 19024, Seattle, WA 98109- 1024, USA.
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