Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (773K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (52)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Vogel, J.
Right arrow Articles by Hess, W. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vogel, J.
Right arrow Articles by Hess, W. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 27, Issue 19 3866-3874, Copyright © 1999 by Oxford University Press

ARTICLES

Comparative analysis of splicing of the complete set of chloroplast group II introns in three higher plant mutants

J Vogel, T Borner and WR Hess
Humboldt-Universitat Berlin, Biologisches Institut/Genetik, Chausseestrasse 117, D-10115 Berlin, Germany.

The barley mutant albostrians and the maize mutants crs1 and crs2 are defective in the splicing of various plastid group II introns. By analysing tRNA precursors and several mRNAs not previously examined, the investigation of in vivo splicing defects in these mutants has been completed. The albostrians mutation causes the loss of plastid ribosomes resulting secondarily in a disruption of splicing of all subgroup IIA introns in the chloroplast. Thus MatK, the only putative chloroplast intron-specific maturase of higher plants, might have evolved to function in splicing of multiple introns. We show that in the case of tRNA-Ala(UGC)the first step of splicing is affected, as suggested by the absence of lariat molecules. Thus the plastid-encoded splicing factor lacking in albostrians must participate in the formation of the catalytically active structure. In contrast, a mutation in the nuclear gene crs1 prevents splicing of only one intron but causes specific additional effects as precursor transcripts for tRNA-Ile(GAU), tRNA-Ala(UGC), tRNA-Lys(UUU)and tRNA-Val(UAC), but not tRNA-Gly(UCC), have significantly enhanced steady-state levels in this mutant. Our data provide evidence for a variety of splicing factors and pathways in the chloroplast, some encoded by nuclear and some by chloroplast genes, and possibly for a dual function of some of these factors.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
T. S. Kroeger, K. P. Watkins, G. Friso, K. J. van Wijk, and A. Barkan
A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing
PNAS, March 17, 2009; 106(11): 4537 - 4542.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
Y. Asakura, O. A. Bayraktar, and A. Barkan
Two CRM protein subfamilies cooperate in the splicing of group IIB introns in chloroplasts
RNA, November 1, 2008; 14(11): 2319 - 2332.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Prikryl, K. P. Watkins, G. Friso, K. J. van Wijk, and A. Barkan
A member of the Whirly family is a multifunctional RNA- and DNA-binding protein that is essential for chloroplast biogenesis
Nucleic Acids Res., September 1, 2008; 36(16): 5152 - 5165.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
J. V. Freudenstein and D. M. Senyo
Relationships and evolution of matK in a group of leafless orchids (Corallorhiza and Corallorhizinae; Orchidaceae: Epidendroideae)
Am. J. Botany, April 1, 2008; 95(4): 498 - 505.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Kahlau and R. Bock
Plastid Transcriptomics and Translatomics of Tomato Fruit Development and Chloroplast-to-Chromoplast Differentiation: Chromoplast Gene Expression Largely Serves the Production of a Single Protein
PLANT CELL, April 1, 2008; 20(4): 856 - 874.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Asakura and A. Barkan
A CRM Domain Protein Functions Dually in Group I and Group II Intron Splicing in Land Plant Chloroplasts
PLANT CELL, December 1, 2007; 19(12): 3864 - 3875.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. F. de Longevialle, E. H. Meyer, C. Andres, N. L. Taylor, C. Lurin, A. H. Millar, and I. D. Small
The Pentatricopeptide Repeat Gene OTP43 Is Required for trans-Splicing of the Mitochondrial nad1 Intron 1 in Arabidopsis thaliana
PLANT CELL, October 1, 2007; 19(10): 3256 - 3265.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
M. M. Barthet and K. W. Hilu
Expression of matK: functional and evolutionary implications
Am. J. Botany, August 1, 2007; 94(8): 1402 - 1412.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. P. Watkins, T. S. Kroeger, A. M. Cooke, R. E. Williams-Carrier, G. Friso, S. E. Belcher, K. J. van Wijk, and A. Barkan
A Ribonuclease III Domain Protein Functions in Group II Intron Splicing in Maize Chloroplasts
PLANT CELL, August 1, 2007; 19(8): 2606 - 2623.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. J. Ostheimer, M. Rojas, H. Hadjivassiliou, and A. Barkan
Formation of the CRS2-CAF2 Group II Intron Splicing Complex Is Mediated by a 22-Amino Acid Motif in the COOH-terminal Region of CAF2
J. Biol. Chem., February 24, 2006; 281(8): 4732 - 4738.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
G. Hausner, R. Olson, D. Simon, I. Johnson, E. R. Sanders, K. G. Karol, R. M. McCourt, and S. Zimmerly
Origin and Evolution of the Chloroplast trnK (matK) Intron: A Model for Evolution of Group II Intron RNA Structures
Mol. Biol. Evol., February 1, 2006; 23(2): 380 - 391.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Meng, Y. Wang, and X.-Q. Liu
An Intron-encoded Protein Assists RNA Splicing of Multiple Similar Introns of Different Bacterial Genes
J. Biol. Chem., October 21, 2005; 280(42): 35085 - 35088.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
O. Ostersetzer, A. M. Cooke, K. P. Watkins, and A. Barkan
CRS1, a Chloroplast Group II Intron Splicing Factor, Promotes Intron Folding through Specific Interactions with Two Intron Domains
PLANT CELL, January 1, 2005; 17(1): 241 - 255.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
P. Jarvinen, A. Palme, L. Orlando Morales, M. Lannenpaa, M. Keinanen, T. Sopanen, and M. Lascoux
Phylogenetic relationships of Betula species (Betulaceae) based on nuclear ADH and chloroplast matK sequences
Am. J. Botany, November 1, 2004; 91(11): 1834 - 1845.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
H. Sugimoto, K. Kusumi, Y. Tozawa, J. Yazaki, N. Kishimoto, S. Kikuchi, and K. Iba
The virescent-2 Mutation Inhibits Translation of Plastid Transcripts for the Plastid Genetic System at an Early Stage of Chloroplast Differentiation
Plant Cell Physiol., August 15, 2004; 45(8): 985 - 996.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
O. W. ODOM, D. L. SHENKENBERG, J. A. GARCIA, and D. L. HERRIN
A horizontally acquired group II intron in the chloroplast psbA gene of a psychrophilic Chlamydomonas: In vitro self-splicing and genetic evidence for maturase activity
RNA, July 1, 2004; 10(7): 1097 - 1107.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
H.-R. Huang, M. Y. Chao, B. Armstrong, Y. Wang, A. M. Lambowitz, and P. S. Perlman
The DIVa Maturase Binding Site in the Yeast Group II Intron aI2 Is Essential for Intron Homing but Not for In Vivo Splicing
Mol. Cell. Biol., December 1, 2003; 23(23): 8809 - 8819.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G. Mohr and A. M. Lambowitz
Putative proteins related to group II intron reverse transcriptase/maturases are encoded by nuclear genes in higher plants
Nucleic Acids Res., January 15, 2003; 31(2): 647 - 652.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
S. A. Kelchner
Group II introns as phylogenetic tools: structure, function, and evolutionary constraints
Am. J. Botany, October 1, 2002; 89(10): 1651 - 1669.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Zimmerly, G. Hausner, and X.-c. Wu
Phylogenetic relationships among group II intron ORFs
Nucleic Acids Res., March 1, 2001; 29(5): 1238 - 1250.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Ohtani, H. Yamamoto, and K. Akimitsu
Sensitivity to Alternaria alternata toxin in citrus because of altered mitochondrial RNA processing
PNAS, February 19, 2002; 99(4): 2439 - 2444.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.