Nucleic Acids Research, Vol 27, Issue 2 455-461, Copyright © 1999 by Oxford University Press
H Wang, E Hartswood and DJ Finnegan
Pogo is a transposable element with short terminal inverted repeats. It
contains two open reading frames that are joined by splicing and code for
the putative pogo transposase, the sequence of which indicates that it is
related to the transposases of members of the Tc1/mariner family as well as
proteins that have no known transposase activity including the centromere
binding protein CENP-B. We have shown that the N- terminal region of pogo
transposase binds in a sequence-specific manner to the ends of pogo and
have identified residues essential for this. The results are consistent
with a prediction that DNA binding is due to a helix-turn-helix motif
within this region. The transposase recognises a 12 bp sequence, two copies
of which are present at each end of pogo DNA. The outer two copies occur as
inverted repeats 14 nucleotides from each end of the element, and contain a
single base mismatch and indicate the inverted repeats of pogo are 26
nucleotides long. The inner copies occur as direct repeats, also with a
single mismatch.
ARTICLES
Pogo transposase contains a putative helix-turn-helix DNA binding domain that recognises a 12 bp sequence within the terminal inverted repeats
Institute of Cell and Molecular Biology, University of Edinburgh, King's Buildings, Edinburgh EH9 3JR, UK.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Loot, N. Santiago, A. Sanz, and J. M. Casacuberta The proteins encoded by the pogo-like Lemi1 element bind the TIRs and subterminal repeated motifs of the Arabidopsis Emigrant MITE: consequences for the transposition mechanism of MITEs Nucleic Acids Res., October 6, 2006; 34(18): 5238 - 5246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Yang, C. F. Weil, and S. R. Wessler A Rice Tc1/Mariner-Like Element Transposes in Yeast PLANT CELL, October 1, 2006; 18(10): 2469 - 2478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. S. Emerald, J. Curtiss, M. Mlodzik, and S. M. Cohen distal antenna and distal antenna related encode nuclear proteins containing pipsqueak motifs involved in antenna development in Drosophila Development, March 15, 2003; 130(6): 1171 - 1180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhang, A. Dawson, and D. J. Finnegan DNA-binding activity and subunit interaction of the mariner transposase Nucleic Acids Res., September 1, 2001; 29(17): 3566 - 3575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Feschotte and C. Mouches Evidence that a Family of Miniature Inverted-Repeat Transposable Elements (MITEs) from the Arabidopsis thaliana Genome Has Arisen from a pogo-like DNA Transposon Mol. Biol. Evol., May 1, 2000; 17(5): 730 - 737. [Abstract] [Full Text] [PDF]
|
|