Nucleic Acids Research, Vol 27, Issue 2 491-495, Copyright © 1999 by Oxford University Press
M Hofferer, C Wirbelauer, B Humar and W Krek
In mammalian cells, DNA damage induces robust changes in gene expression
and these changes contribute to the proper execution of cellular responses
to DNA damage, including DNA repair, cell cycle arrest and apoptosis. The
transcription factor E2F-1 has been suggested to play a key role in the
regulation of cell cycle-dependent gene expression and apoptosis. These
activities depend on the ability of E2F- 1 to form functionally active DNA
binding complexes. Here we describe an assay that allows one to measure
E2F-1 DNA binding activity in naive cells. We find that DNA damage,
generated by UV- or gamma-irradiation, prompts increased production of
E2F-1 DNA binding activity, which, at least in part, originates from
alterations in E2F-1 protein levels. These findings represent an indication
for a role of the transcription factor E2F-1 in the DNA damage response
pathway.
ARTICLES
Increased levels of E2F-1-dependent DNA binding activity after UV- or gamma-irradiation
Friedrich Miescher Institut, Maulbeerstrasse 66, CH-4058 Basel, Switzerland and Departement Forschung, Kantonsspital Basel, Hebelstrasse 20, CH-4031 Basel, Switzerland.
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