Nucleic Acids Research, Vol 27, Issue 2 510-516, Copyright © 1999 by Oxford University Press
Y Takahashi, F Hirose, A Matsukage and M Yamaguchi
The genes for a DNA replication-related element-binding factor (DREF) were
isolated from Drosophila melanogaster and Drosophila virilis, and their
nucleotide sequences were determined. Drosophila virilis DREF consists of
742 amino acid residues, which is 33 amino acids longer than D.melanogaster
DREF. Comparison of the amino acid sequences revealed that D.virilis DREF
is 71% identical to its D. melanogaster homolog. Three highly conserved
regions were identified at amino acid positions 14-182 (CR1), 432-568 (CR2)
and 636-730 (CR3) of the D.virilis DREF, with 86.4, 86.1 and 83.3%
identities, respectively. Transgenic flies in which expression of three
conserved regions of D.melanogaster DREF was targeted to the eye imaginal
disc were established. Expression of CR1 in the developing eye imaginal
discs resulted in a severe rough eye phenotype in adult flies. Expression
of CR3 also caused a rough eye phenotype, while that of CR2 had no apparent
effect on eye morphology. Expression of either CR1 or CR3 in eye imaginal
disc cells inhibited cell cycle progression and reduced incorporation of
5-bromo-2'-deoxyuridine into the S-phase zone (the second mitotic wave)
behind the morphogenetic furrow. The results indicate that both CR1 and CR3
are important for DREF functions.
ARTICLES
Identification of three conserved regions in the DREF transcription factors from Drosophila melanogaster and Drosophila virilis
Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya, Aichi 464-8681, Japan.
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