Nucleic Acids Research, Vol 27, Issue 2 596-600, Copyright © 1999 by Oxford University Press
E Biet, J Sun and M Dutreix
Repetitive sequences have been proposed to be recombinogenic elements in
eukaryotic chromosomes. We tested whether dinucleotide repeats sequences
are preferential sites for recombination because of their high affinity for
recombination enzymes. We compared the kinetics of the binding of the
scRad51, hsRad51 and ecRecA proteins to oligonucleotides with repeats of
dinucleotides GT, CA, CT, GA, GC or AT. Since secondary structures in
single-stranded DNA (ssDNA) act as a barrier to complete binding we
measured whether these oligonucleotides are able to form stable secondary
structures. We show that the preferential binding of recombination proteins
is conserved among the three proteins and is influenced mainly by secondary
structures in ssDNA.
ARTICLES
Conserved sequence preference in DNA binding among recombination proteins: an effect of ssDNA secondary structure
Institut Curie, Section de Recherche UMR144-CNRS, 26 rue d'Ulm, F-75248- Paris Cedex 05, France.
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