Nucleic Acids Research, Vol 27, Issue 3 915-916, Copyright © 1999 by Oxford University Press
PK Koonjul, WF Brandt, JM Farrant and GG Lindsey
Polysaccharides, secondary metabolites and poly-phenolics are known to
co-isolate with nucleic acids from plant tissues resulting in inhibition of
molecular manipulations. RNA isolated from the polyphenolic-rich
resurrection plant, Myrothamnus flabellifolius, was demonstrated to inhibit
a standard polymerase chain reaction used as an assay despite the inclusion
of the polyphenolic-binding compound poly(1- vinylpyrrolidone-2) (PVP) into
the RNA isolation medium. This inhibition was, however, reversed by the
addition of PVP into the PCR mixture itself. Confirmation of the inhibitory
effect of polyphenolics on PCR was obtained by addition of green tea
polyphenolics to the standard PCR assay. This inhibition was also reversed
by the simultaneous inclusion of PVP.
ARTICLES
Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA
Department of Biochemistry and Department of Botany, University of Cape Town, Private Bag, Rondebosch 7701, South Africa.
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